Abstract
The phenoloxidase (PO) activity of the hemocytes lysate supernatant from three ascidians species, assayed by means of 3-methyl-2-benzothiazolinone hydrazone hydrochloride, have been compared. PO-containing hemocytes were identified by a cytochemical reaction and the enzymatic activity measured by a spectrophotometric assay of lysate supernatant from hemocyte populations separated on a discontinuous Percoll density gradient. In Styela plicata, the enzyme appeared to be contained in morula cells only. In Ciona intestinalis, PO activity was shown in univacuolar refractile granulocyte and granular hemocyte. In Phallusia mammillata both compartment cell and granular hemocytes were positive. Enzymatic assay following electrophoretic analysis on polyacrylamide gel electrophoresis (PAGE) or SDS-PAGE indicated that hemocyte lysate presented orthodiphenoloxidase (catecholase) activity. The enzymes from the three species differed in molecular size, activating substances and trypsin sensitivity.
| Original language | English |
|---|---|
| Pages (from-to) | 583-591 |
| Number of pages | 9 |
| Journal | Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology |
| Volume | 135 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Aug 1 2003 |
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Keywords
- Cell separation
- Ciona intestinalis
- Hemocyte
- Immunity
- Phallusia mammillata
- Phenoloxidase
- Styela plicata
- Tunicate
ASJC Scopus subject areas
- Biochemistry
- Physiology
Cite this
Phenoloxidases in ascidian hemocytes : Characterization of the prophenoloxidase activating system. / Parrinello, Nicolò; Arizza, Vincenzo; Chinnici, Cinzia; Parrinello, Daniela; Cammarata, Matteo.
In: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, Vol. 135, No. 4, 01.08.2003, p. 583-591.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Phenoloxidases in ascidian hemocytes
T2 - Characterization of the prophenoloxidase activating system
AU - Parrinello, Nicolò
AU - Arizza, Vincenzo
AU - Chinnici, Cinzia
AU - Parrinello, Daniela
AU - Cammarata, Matteo
PY - 2003/8/1
Y1 - 2003/8/1
N2 - The phenoloxidase (PO) activity of the hemocytes lysate supernatant from three ascidians species, assayed by means of 3-methyl-2-benzothiazolinone hydrazone hydrochloride, have been compared. PO-containing hemocytes were identified by a cytochemical reaction and the enzymatic activity measured by a spectrophotometric assay of lysate supernatant from hemocyte populations separated on a discontinuous Percoll density gradient. In Styela plicata, the enzyme appeared to be contained in morula cells only. In Ciona intestinalis, PO activity was shown in univacuolar refractile granulocyte and granular hemocyte. In Phallusia mammillata both compartment cell and granular hemocytes were positive. Enzymatic assay following electrophoretic analysis on polyacrylamide gel electrophoresis (PAGE) or SDS-PAGE indicated that hemocyte lysate presented orthodiphenoloxidase (catecholase) activity. The enzymes from the three species differed in molecular size, activating substances and trypsin sensitivity.
AB - The phenoloxidase (PO) activity of the hemocytes lysate supernatant from three ascidians species, assayed by means of 3-methyl-2-benzothiazolinone hydrazone hydrochloride, have been compared. PO-containing hemocytes were identified by a cytochemical reaction and the enzymatic activity measured by a spectrophotometric assay of lysate supernatant from hemocyte populations separated on a discontinuous Percoll density gradient. In Styela plicata, the enzyme appeared to be contained in morula cells only. In Ciona intestinalis, PO activity was shown in univacuolar refractile granulocyte and granular hemocyte. In Phallusia mammillata both compartment cell and granular hemocytes were positive. Enzymatic assay following electrophoretic analysis on polyacrylamide gel electrophoresis (PAGE) or SDS-PAGE indicated that hemocyte lysate presented orthodiphenoloxidase (catecholase) activity. The enzymes from the three species differed in molecular size, activating substances and trypsin sensitivity.
KW - Cell separation
KW - Ciona intestinalis
KW - Hemocyte
KW - Immunity
KW - Phallusia mammillata
KW - Phenoloxidase
KW - Styela plicata
KW - Tunicate
UR - http://www.scopus.com/inward/record.url?scp=0043210537&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0043210537&partnerID=8YFLogxK
U2 - 10.1016/S1096-4959(03)00120-9
DO - 10.1016/S1096-4959(03)00120-9
M3 - Article
VL - 135
SP - 583
EP - 591
JO - Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology
SN - 1096-4959
IS - 4
ER -