Phenols removal by immobilized tyrosinase reactor in on-line high performance liquid chromatography

Anna Maria Girelli, Enrico Mattei, Antonella Messina

Research output: Contribution to journalArticlepeer-review


The development of an immobilized enzyme reactor (IMER) based on tyrosinase immobilized on aminopropyl-controlled pore glass (AP-CPG) for the removal of phenols from model aqueous solutions was reported. To elucidate the influence of the substrate nature, the apparent (Vmax, Km) and the inherent (Vmax, Km) Michaelis-Menten constants were determined by Lineweaver-Burk method and the external diffusional contributions on measured enzyme activities were removed by a graphical method. The dephenolization process was realized by recycling the phenol solutions through the bioreactor connected to a chitosan trap in order to remove the colored quinone-type products of the tyrosinase reactions. The results indicated that a complete removal of phenol derivatives in the range of 150-300 min, with the exception of 60% removal for phenol reached in 400 min, was obtained. The observed sequence: cresol > 4-methylcathecol > catechol > 4-Cl-phenol ≫ phenol was in accordance to the Vmax/Km values.

Original languageEnglish
Pages (from-to)271-277
Number of pages7
JournalAnalytica Chimica Acta
Issue number2
Publication statusPublished - Nov 24 2006


  • Bioreactor
  • Phenols removal
  • Tyrosinase immobilized

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry


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