Phenols removal by immobilized tyrosinase reactor in on-line high performance liquid chromatography

Anna Maria Girelli, Enrico Mattei, Antonella Messina

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The development of an immobilized enzyme reactor (IMER) based on tyrosinase immobilized on aminopropyl-controlled pore glass (AP-CPG) for the removal of phenols from model aqueous solutions was reported. To elucidate the influence of the substrate nature, the apparent (Vmax, Km) and the inherent (Vmax, Km) Michaelis-Menten constants were determined by Lineweaver-Burk method and the external diffusional contributions on measured enzyme activities were removed by a graphical method. The dephenolization process was realized by recycling the phenol solutions through the bioreactor connected to a chitosan trap in order to remove the colored quinone-type products of the tyrosinase reactions. The results indicated that a complete removal of phenol derivatives in the range of 150-300 min, with the exception of 60% removal for phenol reached in 400 min, was obtained. The observed sequence: cresol > 4-methylcathecol > catechol > 4-Cl-phenol ≫ phenol was in accordance to the Vmax/Km values.

Original languageEnglish
Pages (from-to)271-277
Number of pages7
JournalAnalytica Chimica Acta
Volume580
Issue number2
DOIs
Publication statusPublished - Nov 24 2006

Fingerprint

Monophenol Monooxygenase
Phenols
High performance liquid chromatography
Phenol
liquid chromatography
phenol
High Pressure Liquid Chromatography
cresol
Immobilized Enzymes
Chitosan
Recycling
Enzyme activity
Bioreactors
graphical method
Glass
bioreactor
enzyme activity
reactor
removal
Derivatives

Keywords

  • Bioreactor
  • Phenols removal
  • Tyrosinase immobilized

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

Phenols removal by immobilized tyrosinase reactor in on-line high performance liquid chromatography. / Girelli, Anna Maria; Mattei, Enrico; Messina, Antonella.

In: Analytica Chimica Acta, Vol. 580, No. 2, 24.11.2006, p. 271-277.

Research output: Contribution to journalArticle

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