TY - JOUR
T1 - Phenotype analysis of unstimulated lymphocytes and anti-cd3-stimulated proliferating t-cells from cerebrospinal fluid and peripheral blood in patients with multiple sclerosis and other neurological diseases
AU - Dufour, A.
AU - Salmaggi, A.
AU - Eoli, M.
AU - Mantia, L. La
AU - Milanese, C.
AU - Nespolo, A.
PY - 1993
Y1 - 1993
N2 - In 15 patients with multiple sclerosis (MS) and in 11 patients with other neurological diseases (OND), the phenotype of fresh unstimulated CSF and PB mononuclear cells and of in vitro" expanded T-cells was studied by monoclonal antibody stain and cytofluorimeter analysis. A compartment-specific decrease of CD8+Leu8+ and CD8+Leu8- cells in CSF was detected; moreover, lower levels of CD8+Leu8- cells were seen in MS than in OND patients, both in CSF and in PB. Although the percentages of unstimulated CSF CD4+ cells did not differ between MS and OND, a higher proportion of in vitro" expanded CD4+ T-cells was obtained from MS patients than from OND. Among MS patients, T-cell growth was very scarce or absent in those sampled during relapses. The results suggest alterations both within the CD4+ helper" and the CD8+ suppressor-cytotoxic" populations in the CSF of MS patients, and stress the relevance of functional analysis in conjunction with phenotype studies.
AB - In 15 patients with multiple sclerosis (MS) and in 11 patients with other neurological diseases (OND), the phenotype of fresh unstimulated CSF and PB mononuclear cells and of in vitro" expanded T-cells was studied by monoclonal antibody stain and cytofluorimeter analysis. A compartment-specific decrease of CD8+Leu8+ and CD8+Leu8- cells in CSF was detected; moreover, lower levels of CD8+Leu8- cells were seen in MS than in OND patients, both in CSF and in PB. Although the percentages of unstimulated CSF CD4+ cells did not differ between MS and OND, a higher proportion of in vitro" expanded CD4+ T-cells was obtained from MS patients than from OND. Among MS patients, T-cell growth was very scarce or absent in those sampled during relapses. The results suggest alterations both within the CD4+ helper" and the CD8+ suppressor-cytotoxic" populations in the CSF of MS patients, and stress the relevance of functional analysis in conjunction with phenotype studies.
KW - Cerebrospinal fluid
KW - Multiple sclerosis
KW - T-cell proliferation
KW - T-cell subsets
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U2 - 10.3109/00207459308986676
DO - 10.3109/00207459308986676
M3 - Article
C2 - 7513316
AN - SCOPUS:0027762221
VL - 73
SP - 277
EP - 285
JO - International Journal of Neuroscience
JF - International Journal of Neuroscience
SN - 0020-7454
IS - 3-4
ER -