Phenotype and homing of CD4 tumor-specific T cells is modulated by tumor bulk

Fabio Benigni, Valérie S. Zimmermann, Stephanie Hugues, Stefano Caserta, Veronica Basso, Laura Rivino, Elizabeth Ingulli, Laurent Malherbe, Nicolas Glaichenhaus, Anna Mondino

Research output: Contribution to journalArticle

Abstract

Technical difficulties in tracking endogenous CD4 T lymphocytes have limited the characterization of tumor-specific CD4 T cell responses. Using fluorescent MHC class II/peptide multimers, we defined the fate of endogenous Leishmania receptor for activated C kinase (LACK)-specific CD4 T cells in mice bearing LACK-expressing TS/A tumors. LACK-specific CD44highCD62L low CD4 T cells accumulated in the draining lymph nodes and had characteristics of effector cells, secreting IL-2 and IFN-γ upon Ag restimulation. Increased frequencies of CD44highCD62Llow LACK-experienced cells were also detected in the spleen, lung, liver, and tumor itself, but not in nondraining lymph nodes, where the cells maintained a naive phenotype. The absence of systemic redistribution of LACK-specific memory T cells correlated with the presence of tumor. Indeed, LACK-specific CD4 T cells with central memory features (IL-2+IFN-γ-CD44 highCD62Lhigh cells) accumulated in all peripheral lymph nodes of mice immunized with LACK-pulsed dendritic cells and after tumor resection. Together, our data demonstrate that although tumor-specific CD4 effector T cells producing IFN-γ are continuously generated in the presence of tumor, central memory CD4 T cells accumulate only after tumor resection. Thus, the continuous stimulation of tumor-specific CD4 T cells in tumor-bearing mice appears to hinder the systemic accumulation of central memory CD4 T lymphocytes.

Original languageEnglish
Pages (from-to)739-748
Number of pages10
JournalJournal of Immunology
Volume175
Issue number2
Publication statusPublished - Jul 15 2005

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Leishmania
T-Lymphocytes
Phenotype
Neoplasms
Lymph Nodes
Interleukin-2
receptors for activated C kinase
Dendritic Cells
Spleen
Lung
Peptides
Liver

ASJC Scopus subject areas

  • Immunology

Cite this

Benigni, F., Zimmermann, V. S., Hugues, S., Caserta, S., Basso, V., Rivino, L., ... Mondino, A. (2005). Phenotype and homing of CD4 tumor-specific T cells is modulated by tumor bulk. Journal of Immunology, 175(2), 739-748.

Phenotype and homing of CD4 tumor-specific T cells is modulated by tumor bulk. / Benigni, Fabio; Zimmermann, Valérie S.; Hugues, Stephanie; Caserta, Stefano; Basso, Veronica; Rivino, Laura; Ingulli, Elizabeth; Malherbe, Laurent; Glaichenhaus, Nicolas; Mondino, Anna.

In: Journal of Immunology, Vol. 175, No. 2, 15.07.2005, p. 739-748.

Research output: Contribution to journalArticle

Benigni, F, Zimmermann, VS, Hugues, S, Caserta, S, Basso, V, Rivino, L, Ingulli, E, Malherbe, L, Glaichenhaus, N & Mondino, A 2005, 'Phenotype and homing of CD4 tumor-specific T cells is modulated by tumor bulk', Journal of Immunology, vol. 175, no. 2, pp. 739-748.
Benigni F, Zimmermann VS, Hugues S, Caserta S, Basso V, Rivino L et al. Phenotype and homing of CD4 tumor-specific T cells is modulated by tumor bulk. Journal of Immunology. 2005 Jul 15;175(2):739-748.
Benigni, Fabio ; Zimmermann, Valérie S. ; Hugues, Stephanie ; Caserta, Stefano ; Basso, Veronica ; Rivino, Laura ; Ingulli, Elizabeth ; Malherbe, Laurent ; Glaichenhaus, Nicolas ; Mondino, Anna. / Phenotype and homing of CD4 tumor-specific T cells is modulated by tumor bulk. In: Journal of Immunology. 2005 ; Vol. 175, No. 2. pp. 739-748.
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