Phenotype of the terminal transferase-positive cells in human foetal liver and bone-marrow: Analysis with monoclonal antibodies

A. Bonati, D. Delia, B. Starcich, M. Buscaglia

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Liver and bone-marrow of 20 human foetuses between 15th and 20th week of gestational age were examined. Mononuclear cells were labelled with murine monoclonal antibodies to reveal surface antigens and with a rabbit antiserum to TdT+ for the detection of the nuclear enzyme, using a double colour indirect immunofluorescence technique. The results have revealed that TdT cells express the phenotype of B cell precursors HLA-DR+, CALLA+, FMC8+, LEU-1-, LEU-5-, LEU-9-. It suggests that foetal liver and bone-marrow have an active function in B-lymphopoiesis in humans. The involvement of TdT in the process of B-cell immunological acquisition is uncertain because only humans and Xenopus embryos, among the species examined, express TdT outside the thymus during ontogenesis.

Original languageEnglish
Pages (from-to)418-424
Number of pages7
JournalScandinavian Journal of Haematology
Volume33
Issue number5
Publication statusPublished - 1984

Fingerprint

Transferases
Bone Marrow
Monoclonal Antibodies
Phenotype
Liver
Lymphopoiesis
B-Lymphoid Precursor Cells
HLA-DR Antigens
Surface Antigens
Indirect Fluorescent Antibody Technique
Xenopus
Thymus Gland
Gestational Age
Immune Sera
Fetus
B-Lymphocytes
Embryonic Structures
Color
Rabbits
Enzymes

ASJC Scopus subject areas

  • Hematology

Cite this

Phenotype of the terminal transferase-positive cells in human foetal liver and bone-marrow : Analysis with monoclonal antibodies. / Bonati, A.; Delia, D.; Starcich, B.; Buscaglia, M.

In: Scandinavian Journal of Haematology, Vol. 33, No. 5, 1984, p. 418-424.

Research output: Contribution to journalArticle

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AU - Buscaglia, M.

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