Phenotype of the terminal transferase-positive cells in human foetal liver and bone-marrow: Analysis with monoclonal antibodies

A. Bonati, D. Delia, B. Starcich, M. Buscaglia

Research output: Contribution to journalArticlepeer-review

Abstract

Liver and bone-marrow of 20 human foetuses between 15th and 20th week of gestational age were examined. Mononuclear cells were labelled with murine monoclonal antibodies to reveal surface antigens and with a rabbit antiserum to TdT+ for the detection of the nuclear enzyme, using a double colour indirect immunofluorescence technique. The results have revealed that TdT cells express the phenotype of B cell precursors HLA-DR+, CALLA+, FMC8+, LEU-1-, LEU-5-, LEU-9-. It suggests that foetal liver and bone-marrow have an active function in B-lymphopoiesis in humans. The involvement of TdT in the process of B-cell immunological acquisition is uncertain because only humans and Xenopus embryos, among the species examined, express TdT outside the thymus during ontogenesis.

Original languageEnglish
Pages (from-to)418-424
Number of pages7
JournalScandinavian Journal of Haematology
Volume33
Issue number5
Publication statusPublished - 1984

ASJC Scopus subject areas

  • Hematology

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