Phenotypic analysis of hairy cell leukemia: 'Variant' cases express the interleukin-2 receptor β chain, but not the α chain (CD25)

D. De Totero, P. L. Tazzari, F. Lauria, D. Raspadori, P. F. Di Celle, A. Carbone, M. Gobbi, R. Foa

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Hairy cell leukemia (HCL) is a B-cell chronic lymphoproliferative disorder in which the pathologic cells show a strong expression of CD25 (interleukin- 2 [IL-2] receptor α chain or p55). 'Variant' cases of HCL, characterized by hyperleukocytosis, neoplastic elements with a prominent nucleolus and a higher nucleo/cytoplasmic ratio, and an easily obtained bone marrow aspirate, lack surface CD25 determinants. Limited information is available on the expression of the IL-2 receptor β chain (p75) on normal and neoplastic B cells. In this study, we have assessed by immunofluorescence and mRNA analysis the presence of the IL-2 receptor α and β chains on 12 cases of classic HCL, as well as on 3 variant cases. The results obtained show that, while the α chain of the IL-2 receptor is present only on classic HCL, the IL-2 receptor β chain (p75) is expressed on both the classic and variant form. Unlike hairy cells, only 8 of the 15 B-cell chronic lymphocytic leukemia cases tested showed a weak expression of the p75 antigen on a small proportion of cells. Purified B lymphocytes from normal healthy controls, as well as Epstein-Barr virus-transformed lymphoblastoid cell lines, showed a weak staining for the p75 determinant, while being CD25 -. The results of this study suggest that the expression of the α and β chains of the IL-2 receptor appears to be upregulated or downregulated during the process of B- cell-lineage activation and differentiation.

Original languageEnglish
Pages (from-to)528-535
Number of pages8
JournalBlood
Volume82
Issue number2
Publication statusPublished - 1993

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Hairy Cell Leukemia
Interleukin-2 Receptors
Cells
B-Lymphocytes
Transformed Cell Line
Lymphoproliferative Disorders
Lymphocytes
Cell Lineage
B-Cell Chronic Lymphocytic Leukemia
Human Herpesvirus 4
Viruses
Fluorescent Antibody Technique
Cell Differentiation
Bone
Down-Regulation
Bone Marrow
Chemical activation
Staining and Labeling
Antigens
Messenger RNA

ASJC Scopus subject areas

  • Hematology

Cite this

De Totero, D., Tazzari, P. L., Lauria, F., Raspadori, D., Di Celle, P. F., Carbone, A., ... Foa, R. (1993). Phenotypic analysis of hairy cell leukemia: 'Variant' cases express the interleukin-2 receptor β chain, but not the α chain (CD25). Blood, 82(2), 528-535.

Phenotypic analysis of hairy cell leukemia : 'Variant' cases express the interleukin-2 receptor β chain, but not the α chain (CD25). / De Totero, D.; Tazzari, P. L.; Lauria, F.; Raspadori, D.; Di Celle, P. F.; Carbone, A.; Gobbi, M.; Foa, R.

In: Blood, Vol. 82, No. 2, 1993, p. 528-535.

Research output: Contribution to journalArticle

De Totero, D, Tazzari, PL, Lauria, F, Raspadori, D, Di Celle, PF, Carbone, A, Gobbi, M & Foa, R 1993, 'Phenotypic analysis of hairy cell leukemia: 'Variant' cases express the interleukin-2 receptor β chain, but not the α chain (CD25)', Blood, vol. 82, no. 2, pp. 528-535.
De Totero D, Tazzari PL, Lauria F, Raspadori D, Di Celle PF, Carbone A et al. Phenotypic analysis of hairy cell leukemia: 'Variant' cases express the interleukin-2 receptor β chain, but not the α chain (CD25). Blood. 1993;82(2):528-535.
De Totero, D. ; Tazzari, P. L. ; Lauria, F. ; Raspadori, D. ; Di Celle, P. F. ; Carbone, A. ; Gobbi, M. ; Foa, R. / Phenotypic analysis of hairy cell leukemia : 'Variant' cases express the interleukin-2 receptor β chain, but not the α chain (CD25). In: Blood. 1993 ; Vol. 82, No. 2. pp. 528-535.
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AU - Lauria, F.

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AU - Di Celle, P. F.

AU - Carbone, A.

AU - Gobbi, M.

AU - Foa, R.

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