Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS

Debora Giunti, Giovanna Borsellino, Roberto Benelli, Monica Marchese, Elisabetta Capello, Maria Teresa Valle, Enrico Pedemonte, Douglas Noonan, Adriana Albini, Giorgio Bernardi, Giovanni Luigi Mancardi, Luca Battistini, Antonio Uccelli

Research output: Contribution to journalArticle

Abstract

The recruitment of lymphocytes across the blood brain barrier (BBB) is mediated by adhesion molecules and chemokines. The expression of activation markers and of chemokine receptors on T cells homing to the nervous system (NS) may help define their functional state. In the cerebrospinal fluid (CSF) of subjects with inflammatory neurological diseases (IND), including multiple sclerosis, we observed an increased number of T cells coexpressing CXCR3 and CCR5 as well as T cells with a CD45RO+ CCR7+ CD27+ memory phenotype. A subset of CCR7+ T cells coexpressed CXCR3 and CCR5. We also detected an increased number of interferon-γ-producing T cells in the CSF compared with peripheral blood, mostly but not exclusively in the CD45RO+ CCR72- CD27-compartment. T helper 1 (Th1) clones, established from the CSF of individuals with IND and from a healthy subject, similarly migrated to CXCL10, CXCL12, and CCL5. CXCL10, CXCL12, and CCL19 were increased in the CSF of individuals with neuroinflammation. These findings suggest that CSF is enriched in Th1-polarized memory T cells capable of differentiating into effector cells upon antigen encounter. These cells are recruited into the CSF by inducible chemokines. Thus, CSF represents a transitional station for T cells trafficking to and from the NS.

Original languageEnglish
Pages (from-to)584-590
Number of pages7
JournalJournal of Leukocyte Biology
Volume73
Issue number5
DOIs
Publication statusPublished - May 2003

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Central Nervous System Diseases
Cerebrospinal Fluid
T-Lymphocytes
Chemokines
Nervous System
Chemokine Receptors
T-Lymphocyte Subsets
Blood-Brain Barrier
Interferons
Multiple Sclerosis
Healthy Volunteers
Clone Cells
Lymphocytes
Phenotype
Antigens

Keywords

  • Cell trafficking
  • Chemokines
  • MS
  • Neuroimmunology
  • T lymphocytes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS. / Giunti, Debora; Borsellino, Giovanna; Benelli, Roberto; Marchese, Monica; Capello, Elisabetta; Valle, Maria Teresa; Pedemonte, Enrico; Noonan, Douglas; Albini, Adriana; Bernardi, Giorgio; Mancardi, Giovanni Luigi; Battistini, Luca; Uccelli, Antonio.

In: Journal of Leukocyte Biology, Vol. 73, No. 5, 05.2003, p. 584-590.

Research output: Contribution to journalArticle

Giunti, Debora ; Borsellino, Giovanna ; Benelli, Roberto ; Marchese, Monica ; Capello, Elisabetta ; Valle, Maria Teresa ; Pedemonte, Enrico ; Noonan, Douglas ; Albini, Adriana ; Bernardi, Giorgio ; Mancardi, Giovanni Luigi ; Battistini, Luca ; Uccelli, Antonio. / Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS. In: Journal of Leukocyte Biology. 2003 ; Vol. 73, No. 5. pp. 584-590.
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