TY - JOUR
T1 - Phenotypic and functional characteristics of tumor-associated lymphocytes in patients with malignant ascites receiving intraperitoneal infusions of recombinant interleukin-2 (rIL-2)
AU - Melioli, G.
AU - Baldini, E.
AU - Mingari, M. C.
AU - De Maria, A.
AU - Sertoli, M. R.
AU - Badellino, F.
AU - Percivale, P. L.
AU - Catturich, A.
AU - Bertoglio, S.
AU - Civalleri, D.
AU - Santi, L.
AU - Moretta, L.
PY - 1989
Y1 - 1989
N2 - In the course of a phase I trial, in which recombinant IL-2 (rIL-2) was infused intraperitoneally (i.p.) in patients with peritoneal carcinomatosis, we evaluated the effect on 'tumor-associated lymphocytes' (TAL) isolated from the ascitic fluid. No major changes in the percentages of cells expressing the CD3, CD4, CD8, Leu-7, OKMI, and WT-31 antigens were detected either in TAL or in peripheral blood lymphocytes (PBL) after 7 days of rIL-2 infusion. In contrast the percentages of TAL (but not PBL) expressing surface IL-2 receptor (Tac), or LAK-1 antigen were sharply increased. Analysis of cytolytic functions showed a potentiation of the lytic activity against natural-killer (NK) sensitive K562 target cells and the de novo appearance of lytic activity against fresh melanoma cells. In one patient IFN-γ was detected in the ascitic fluid following rIL-2 infusion. T-cell clones derived from the patient were analyzed for the IFN-γ production. While only approximately 40% of PB-derived control clones produced medium to low amounts of IFN-γ, all of the TAL-derived clones produced medium to high amounts of the lymphokine.
AB - In the course of a phase I trial, in which recombinant IL-2 (rIL-2) was infused intraperitoneally (i.p.) in patients with peritoneal carcinomatosis, we evaluated the effect on 'tumor-associated lymphocytes' (TAL) isolated from the ascitic fluid. No major changes in the percentages of cells expressing the CD3, CD4, CD8, Leu-7, OKMI, and WT-31 antigens were detected either in TAL or in peripheral blood lymphocytes (PBL) after 7 days of rIL-2 infusion. In contrast the percentages of TAL (but not PBL) expressing surface IL-2 receptor (Tac), or LAK-1 antigen were sharply increased. Analysis of cytolytic functions showed a potentiation of the lytic activity against natural-killer (NK) sensitive K562 target cells and the de novo appearance of lytic activity against fresh melanoma cells. In one patient IFN-γ was detected in the ascitic fluid following rIL-2 infusion. T-cell clones derived from the patient were analyzed for the IFN-γ production. While only approximately 40% of PB-derived control clones produced medium to low amounts of IFN-γ, all of the TAL-derived clones produced medium to high amounts of the lymphokine.
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M3 - Article
C2 - 2492978
AN - SCOPUS:0024514158
VL - 43
SP - 231
EP - 234
JO - International Journal of Cancer
JF - International Journal of Cancer
SN - 0020-7136
IS - 2
ER -