Phenotypic and functional heterogeneity of human T cell clones producing γ-interferon

Giovanni Melioli, Annalisa Merli, Silvano Ferrini, Maria Cristina Mingari, Lorenzo Moretta

Research output: Contribution to journalArticle

Abstract

Human T cell clones were derived from peripheral blood and screened for their ability to release γ-interferon (γ-IF) following PHA stimulation. Nine clones producing more than 20 U/ml of γ-IF were expanded and analyzed for cytolytic activity in a lectin-dependent assay and for the ability to release interleukin-2 (IL-2). In addition, clones were analyzed for T4 and T8 antigen expression. Five out of nine clones had cytolytic T lymphocyte activity, while four released relatively large amounts of IL-2. Five clones were T4+ while the remaining expressed the T8+ phenotype. These results clearly indicate that γ-IF production is not restricted to T cell subsets defined according to either functional or phenotypic criteria.

Original languageEnglish
Pages (from-to)47-52
Number of pages6
JournalLa Ricerca in Clinica e in Laboratorio
Volume15
Issue number1
DOIs
Publication statusPublished - Jan 1985

Keywords

  • γ-interferon
  • Cytolytic T lymphocytes
  • Interleukin-2
  • T cell clones
  • T4/T8 antigens

ASJC Scopus subject areas

  • Clinical Biochemistry

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