Phenotypic changes of p53, HER2, and FAS system in multiple normal tissues surrounding breast cancer

Marcella Mottolese, Edit A. Nádasi, Claudio Botti, Anna M. Cianciulli, Roberta Merola, Simonetta Buglioni, Maria Benevolo, Diana Giannarelli, Ferdinando Marandino, Raffaele Perrone Donnorso, Irene Venturo, Pier Giorgio Natali

Research output: Contribution to journalArticlepeer-review


To determine whether phenotypic field changes occur in tissues adjacent to carcinoma, we assayed, by immunohistochemistry, the expression of HER-2, p53, Fas, and FasL in 72 breast cancers (BC) and multiple autologous peritumoral tissues (PTTs) sampled up to 5 cm distance and in 44 benign breast tumors (BBTs). About 5% and 3% of the PTTs and 4.5% and 6.8% of BBTs showed alterations in HER2 and p53 expression, respectively. Of interest, gene amplification was observed in 50% of HER2 positive PTTs, but not in any HER2 positive BBTs. Fas, highly expressed in BBTs and downregulated in BC, maintained its expression in PTTs, whereas FasL, usually negative in BBTs, was upregulated in BC as well as in the PTTs closest (1 cm) to the invasive lesion. Our data suggest that FasL could be a potential novel biomarker of transformation, which may identify, along with HER2 and p53, precursor lesions in a genetically altered breast tissue.

Original languageEnglish
Pages (from-to)106-112
Number of pages7
JournalJournal of Cellular Physiology
Issue number1
Publication statusPublished - Jul 2005

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology


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