Phenotypic characterization of human cytolytic T lymphocytes in mixed lymphocyte culture

A. Moretta, M. C. Mingari, B. F. Haynes, R. P. Sekaly, L. Moretta, A. S. Fauci

Research output: Contribution to journalArticlepeer-review

Abstract

Mixed lymphocyte reaction (MLR)-activated T cells were analyzed according to the expression of various cell surface markers by the specific cytotoxic T lymphocytes (CTL) generated in the MLR. CTL were found exclusively in a population of MLR-activated T cells that lacked detectable FcγR but that expressed a surface antigen recognized by the 4F 2 monoclonal antibody. In contrast, CTL were found in both the Ia-positive and Ia-negative cells after MLR activation. Thus, the specific CTL generated in the allogenic MLR can be identified and isolated by virtue of the expression of a particular cell surface marker.

Original languageEnglish
Pages (from-to)213-218
Number of pages6
JournalJournal of Experimental Medicine
Volume153
Issue number1
DOIs
Publication statusPublished - 1981

ASJC Scopus subject areas

  • Immunology

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