Phenotypic diversity in human fibroblasts from myelometaplasic and non- myelometaplasic hematopoietic tissues

Danièle Brouty-Boyeŕ, Christelle Doucet, Denis Clay, Marie Caroline Le Bousse-Kerdiles, Théodore J. Lampidis, Bruno Azzarone

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Fibroblasts from a variety of tissues interact with and influence the behavior of the cell types they are associated with by producing specific proteins that mediate these interactions. Thus, it is not surprising that fibroblasts have been shown to differ phenotypically and functionally depending on the tissue they are isolated from and its physiologic state. To study fibroblasts of hematopoietic tissues, cultures were established from human normal bone marrow (BM), and from non-myelometaplasic (NS) and myelometaplasic spleen (MMS) tissues and analyzed for phenotypic characteristics. The results are summarized as follows: (1) cytoskeletal elements: virtually all the MMS fibroblasts were stained positively for α- sm-actin while only a small fraction of BM and of NS fibroblasts were positive for this antigen; (2) extracellular matrix elements: MMS fibroblasts stained positively for ED-B fibronectin and tenascin while the other 2 fibroblast cell types did not; (3) cell surface molecules: NS and MMS fibroblasts expressed significantly higher levels of ICAM-1, VLA-4 and CD9 than BM fibroblasts. Moreover, MMS fibroblasts showed a higher expression of ICAM-1 and VLA-4 than NS fibroblasts; and (4) cytokines: IL-11, RANTES and MIP-1α were produced in higher amounts by BM than by NS fibroblasts. Conversely, production of GM-CSF, SCF, M-CSF and MCP-1α was elevated in NS compared with BM fibroblasts. The production of these cytokines was generally reduced in MMS cells. Overall, our results demonstrate that phenotypic characteristics can be identified to distinguish fibroblasts from normal and pathologic hematopoietic tissues. Such phenotypic characteristics suggest functional differences of each type of fibroblast in their influence on the blood cells with which they are associated.

Original languageEnglish
Pages (from-to)767-773
Number of pages7
JournalInternational Journal of Cancer
Volume76
Issue number5
DOIs
Publication statusPublished - 1998

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Fibroblasts
Spleen
Bone Marrow
Macrophage Colony-Stimulating Factor
Integrin alpha4beta1
Intercellular Adhesion Molecule-1
Interleukin-11
Cytokines
Tenascin
Chemokine CCL5
Granulocyte-Macrophage Colony-Stimulating Factor
Fibronectins
Extracellular Matrix
Actins
Blood Cells
Antigens

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Phenotypic diversity in human fibroblasts from myelometaplasic and non- myelometaplasic hematopoietic tissues. / Brouty-Boyeŕ, Danièle; Doucet, Christelle; Clay, Denis; Le Bousse-Kerdiles, Marie Caroline; Lampidis, Théodore J.; Azzarone, Bruno.

In: International Journal of Cancer, Vol. 76, No. 5, 1998, p. 767-773.

Research output: Contribution to journalArticle

Brouty-Boyeŕ, Danièle ; Doucet, Christelle ; Clay, Denis ; Le Bousse-Kerdiles, Marie Caroline ; Lampidis, Théodore J. ; Azzarone, Bruno. / Phenotypic diversity in human fibroblasts from myelometaplasic and non- myelometaplasic hematopoietic tissues. In: International Journal of Cancer. 1998 ; Vol. 76, No. 5. pp. 767-773.
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