Phenotypic, functional and molecular analysis of CD3- LGL expansions indicates a relationship to two different CD3- normal counterparts

C. Cantoni, D. De Totero, F. Lauria, D. Raspadori, R. Conte, S. Ferrini, P. L. Tazzari, R. Biassoni

Research output: Contribution to journalArticle

Abstract

In this study we have analysed the CD3 and TCR transcript expression in three CD3- large granular lymphocyte (LGL) expansions. These LGL populations show a heterogenous pattern of expression for CD2, CD8, CD16, CD56 and CD57 antigens. LGL1 is CD2+CD8-CD16+CD56+CD57+, while LGL2 is CD2- CD8+CD16-CD56-CD57-; LGL3 is similar to LGL1, except for CD8 antigen expression. Functional analysis has revealed a different behaviour of these LGL expansions in a cytotoxicity assay against the NK-sensitive K562 cell line. LGL1 and 3 display a significant NK-like activity, while LGL2 is inefficient against K562 target cells. TCR and CD3 transcript characterization of LGL expansions 1 and 3 showed that they expressed multiple TCRδ transcript, a non-functional TCRβ transcript, CD3-ζ and -ε mRNA, but they lacked or expressed at very low levels of CD3γ transcript. On the other hand, LGL2 expressed TCRδ, CD3-γ, -ε, and -ζ transcripts, while it lacked CD3δ mRNA. On the basis of these data, LGL1 and 3 seem to be closely related to peripheral blood mature natural killer (NK) cells, whereas LGL2 displays a pattern of TCR and CD3 expression similar to that found in CD1-2-3-4-8-16- thymocytes.

Original languageEnglish
Pages (from-to)740-745
Number of pages6
JournalBritish Journal of Haematology
Volume86
Issue number4
Publication statusPublished - 1994

Keywords

  • CD3 LGL expansions
  • gene expression
  • NK cells
  • thymocytes

ASJC Scopus subject areas

  • Hematology

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