In this study we have analysed the CD3 and TCR transcript expression in three CD3- large granular lymphocyte (LGL) expansions. These LGL populations show a heterogenous pattern of expression for CD2, CD8, CD16, CD56 and CD57 antigens. LGL1 is CD2+CD8-CD16+CD56+CD57+, while LGL2 is CD2- CD8+CD16-CD56-CD57-; LGL3 is similar to LGL1, except for CD8 antigen expression. Functional analysis has revealed a different behaviour of these LGL expansions in a cytotoxicity assay against the NK-sensitive K562 cell line. LGL1 and 3 display a significant NK-like activity, while LGL2 is inefficient against K562 target cells. TCR and CD3 transcript characterization of LGL expansions 1 and 3 showed that they expressed multiple TCRδ transcript, a non-functional TCRβ transcript, CD3-ζ and -ε mRNA, but they lacked or expressed at very low levels of CD3γ transcript. On the other hand, LGL2 expressed TCRδ, CD3-γ, -ε, and -ζ transcripts, while it lacked CD3δ mRNA. On the basis of these data, LGL1 and 3 seem to be closely related to peripheral blood mature natural killer (NK) cells, whereas LGL2 displays a pattern of TCR and CD3 expression similar to that found in CD1-2-3-4-8-16- thymocytes.
|Number of pages||6|
|Journal||British Journal of Haematology|
|Publication status||Published - 1994|
- CD3 LGL expansions
- gene expression
- NK cells
ASJC Scopus subject areas