Abstract
Cytokine mobilized peripheral blood progenitor cells are widely used lor iranspl.ini.iiu'n in cancer patients because of easy collection and rapid hématologie reconstitution These cells may be appropriate targets for gene transfer. Therefore, We investigated the effects of ex vivo manipulation on hematopoietic reconstitution potential of these cells. Immunoselected CD34± progenitors isolated from steady-state (ssCD34±) or G-CSF mobilized (mCD34±) peripheral blood were cultured for 5 days: 2-day liquid culture followed by 3-day coculture on A7.21, a rnurine fibroblast layer, in the presence of 10% PCS, 1L-3, IL-6, GM-CSF, and KL. Higher levels of expansion in CD34± cells were observed in ssCD34± than in mCD34± cells (4.6±3.3- vs. 2.2±1.3-fold). Hematopoietic reconstitution potentials of these ex vivo manipulated cells were evaluated using the SCID-hu mouse models implanted with human fetal bone marrow (BM) or thymus/liver. Human BM analyzed 4 weeks after injection of manipulated ssCD34± cells showed reconstitution in CD19± (0.6-98.8%), CD33± (2.8-99.3%) and CD34± progenitor (2.4-17%) cells (n=4); ihyinic grafts analyzed 6-8 weeks after injection also demonstrated high levels of T cells reconstitution (67.2-86.4%) (n=4). In contrast, only very low levels of reconstitution (
| Original language | English |
|---|---|
| Pages (from-to) | 879 |
| Number of pages | 1 |
| Journal | Experimental Hematology |
| Volume | 25 |
| Issue number | 8 |
| Publication status | Published - 1997 |
Fingerprint
ASJC Scopus subject areas
- Cancer Research
- Cell Biology
- Genetics
- Hematology
- Oncology
- Transplantation
Cite this
Phenotypical and functional characterizations of ex vivo manipulated cd34± progenitors from steady state and mobilized peripheral blood. / Humeau, L.; Namikawa, R.; Bardin, F.; Maroc, C.; Manonni, P.; Roncarolo, M. G.; Chabannon, C.
In: Experimental Hematology, Vol. 25, No. 8, 1997, p. 879.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Phenotypical and functional characterizations of ex vivo manipulated cd34± progenitors from steady state and mobilized peripheral blood
AU - Humeau, L.
AU - Namikawa, R.
AU - Bardin, F.
AU - Maroc, C.
AU - Manonni, P.
AU - Roncarolo, M. G.
AU - Chabannon, C.
PY - 1997
Y1 - 1997
N2 - Cytokine mobilized peripheral blood progenitor cells are widely used lor iranspl.ini.iiu'n in cancer patients because of easy collection and rapid hématologie reconstitution These cells may be appropriate targets for gene transfer. Therefore, We investigated the effects of ex vivo manipulation on hematopoietic reconstitution potential of these cells. Immunoselected CD34± progenitors isolated from steady-state (ssCD34±) or G-CSF mobilized (mCD34±) peripheral blood were cultured for 5 days: 2-day liquid culture followed by 3-day coculture on A7.21, a rnurine fibroblast layer, in the presence of 10% PCS, 1L-3, IL-6, GM-CSF, and KL. Higher levels of expansion in CD34± cells were observed in ssCD34± than in mCD34± cells (4.6±3.3- vs. 2.2±1.3-fold). Hematopoietic reconstitution potentials of these ex vivo manipulated cells were evaluated using the SCID-hu mouse models implanted with human fetal bone marrow (BM) or thymus/liver. Human BM analyzed 4 weeks after injection of manipulated ssCD34± cells showed reconstitution in CD19± (0.6-98.8%), CD33± (2.8-99.3%) and CD34± progenitor (2.4-17%) cells (n=4); ihyinic grafts analyzed 6-8 weeks after injection also demonstrated high levels of T cells reconstitution (67.2-86.4%) (n=4). In contrast, only very low levels of reconstitution (
AB - Cytokine mobilized peripheral blood progenitor cells are widely used lor iranspl.ini.iiu'n in cancer patients because of easy collection and rapid hématologie reconstitution These cells may be appropriate targets for gene transfer. Therefore, We investigated the effects of ex vivo manipulation on hematopoietic reconstitution potential of these cells. Immunoselected CD34± progenitors isolated from steady-state (ssCD34±) or G-CSF mobilized (mCD34±) peripheral blood were cultured for 5 days: 2-day liquid culture followed by 3-day coculture on A7.21, a rnurine fibroblast layer, in the presence of 10% PCS, 1L-3, IL-6, GM-CSF, and KL. Higher levels of expansion in CD34± cells were observed in ssCD34± than in mCD34± cells (4.6±3.3- vs. 2.2±1.3-fold). Hematopoietic reconstitution potentials of these ex vivo manipulated cells were evaluated using the SCID-hu mouse models implanted with human fetal bone marrow (BM) or thymus/liver. Human BM analyzed 4 weeks after injection of manipulated ssCD34± cells showed reconstitution in CD19± (0.6-98.8%), CD33± (2.8-99.3%) and CD34± progenitor (2.4-17%) cells (n=4); ihyinic grafts analyzed 6-8 weeks after injection also demonstrated high levels of T cells reconstitution (67.2-86.4%) (n=4). In contrast, only very low levels of reconstitution (
UR - http://www.scopus.com/inward/record.url?scp=33748634370&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33748634370&partnerID=8YFLogxK
M3 - Article
VL - 25
SP - 879
JO - Experimental Hematology
JF - Experimental Hematology
SN - 0301-472X
IS - 8
ER -