Phenotypical characterization of circulating cell subsets in pyoderma gangrenosum patients: The experience of the Italian immuno-pathology group

P. Quaglino, Paolo Fava, M. Caproni, E. Antiga, Clara De Simone, M. Papini, Aurora Parodi, M. Novelli, S. Abate Osella, S. Ribero, M. Sanlorenzo, R. Ponti, M. T. Fierro, Angelo Valerio Marzano, P. Savoia

Research output: Contribution to journalArticlepeer-review

Abstract

Background No data are available as to the phenotype of circulating lymphocyte subsets in pyoderma gangrenosum (PG). Aim To analyse the expression of different chemokine receptors associated to T-helper (Th)1 (CCR5), Th2 (CCR4) and Th17 (CCR6), as well as the regulatory T-cell subset (Treg) and dendritic cell polarization in the blood of newly diagnosed untreated PG patients. Materials and methods Multi-parameter flow cytometry was performed on blood samples from 10 PG patients collected at first diagnosis among centres belonging to the Italian Immuno-pathology Group. Blood samples from 10 age- and sex-matched healthy controls (HC) were used as controls. Results PG patients are characterized by an over-expression in the blood of the CD4+CCR5+ and CD4+CCR6+ and a down-regulation of CD4+CCR4+ counts with respect to healthy subjects. Moreover, they show increased levels of myeloid derived dendritic cells type1 and reduced levels of the Treg CD4+CD25highFOXP3+ subset. Conclusions The pattern of chemokine expression argues in favour of a Th1 (CCR5+) and Th17 (CCR6+) polarization with a down-regulation of Th2 (CCR4+).

Original languageEnglish
Pages (from-to)655-658
Number of pages4
JournalJournal of the European Academy of Dermatology and Venereology
Volume30
Issue number4
DOIs
Publication statusPublished - Apr 1 2016

ASJC Scopus subject areas

  • Dermatology
  • Infectious Diseases

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