Phenotypically immature IgG-bearing B cells in patients with hypogammaglobulinemia

Massimo Fiorilli, Marco Crescenzi, Maurizio Carbonari, Letizia Tedesco, Giandomenico Russo, Carlo Gaetano, Fernando Aiuti

Research output: Contribution to journalArticlepeer-review

Abstract

We investigated the prevalence of phenotypically immature IgG B cells (i.e., coexpressing surface IgG and IgM) in the peripheral blood of 12 patients with hypogammaglobulinemia and in normal individuals. Patients had ataxia-telangiectasia (N=1), hyper-IgM combined immunodeficiency (N=1), or common variable immunodeficiency (CVI). IgG/IgM-positive B cells were evaluated by two-color immunofluorescence using fluoresceinor rhodamine-conjugated goat antiserum; to minimize artifacts due to in vivo cytophilic binding of autologous IgG, cell-bound cytophilic Ig were eluted at pH 4 and Fc receptors were blocked by heat-aggregated rabbit IgG before fluorescent staining. All patients, except two with late-onset CVI, had markedly increased proportions of double-stained IgG B cells (56 to 100% of IgG-bearing B cells) in comparison with normal individuals (11 to 33%).

Original languageEnglish
Pages (from-to)21-25
Number of pages5
JournalJournal of Clinical Immunology
Volume6
Issue number1
DOIs
Publication statusPublished - Jan 1986

Keywords

  • B Cells
  • hypogammaglobulinemia
  • surface immunoglobulins

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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