Phenotypically immature IgG-bearing B cells in patients with hypogammaglobulinemia

Massimo Fiorilli, Marco Crescenzi, Maurizio Carbonari, Letizia Tedesco, Giandomenico Russo, Carlo Gaetano, Fernando Aiuti

Research output: Contribution to journalArticle

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Abstract

We investigated the prevalence of phenotypically immature IgG B cells (i.e., coexpressing surface IgG and IgM) in the peripheral blood of 12 patients with hypogammaglobulinemia and in normal individuals. Patients had ataxia-telangiectasia (N=1), hyper-IgM combined immunodeficiency (N=1), or common variable immunodeficiency (CVI). IgG/IgM-positive B cells were evaluated by two-color immunofluorescence using fluoresceinor rhodamine-conjugated goat antiserum; to minimize artifacts due to in vivo cytophilic binding of autologous IgG, cell-bound cytophilic Ig were eluted at pH 4 and Fc receptors were blocked by heat-aggregated rabbit IgG before fluorescent staining. All patients, except two with late-onset CVI, had markedly increased proportions of double-stained IgG B cells (56 to 100% of IgG-bearing B cells) in comparison with normal individuals (11 to 33%).

Original languageEnglish
Pages (from-to)21-25
Number of pages5
JournalJournal of Clinical Immunology
Volume6
Issue number1
DOIs
Publication statusPublished - Jan 1986

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Agammaglobulinemia
B-Lymphocytes
Immunoglobulin G
Common Variable Immunodeficiency
Immunoglobulin M
Ataxia Telangiectasia
Rhodamines
B-Lymphoid Precursor Cells
Fc Receptors
Goats
Artifacts
Fluorescent Antibody Technique
Immune Sera
Color
Hot Temperature
Staining and Labeling
Rabbits

Keywords

  • B Cells
  • hypogammaglobulinemia
  • surface immunoglobulins

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Fiorilli, M., Crescenzi, M., Carbonari, M., Tedesco, L., Russo, G., Gaetano, C., & Aiuti, F. (1986). Phenotypically immature IgG-bearing B cells in patients with hypogammaglobulinemia. Journal of Clinical Immunology, 6(1), 21-25. https://doi.org/10.1007/BF00915360

Phenotypically immature IgG-bearing B cells in patients with hypogammaglobulinemia. / Fiorilli, Massimo; Crescenzi, Marco; Carbonari, Maurizio; Tedesco, Letizia; Russo, Giandomenico; Gaetano, Carlo; Aiuti, Fernando.

In: Journal of Clinical Immunology, Vol. 6, No. 1, 01.1986, p. 21-25.

Research output: Contribution to journalArticle

Fiorilli, M, Crescenzi, M, Carbonari, M, Tedesco, L, Russo, G, Gaetano, C & Aiuti, F 1986, 'Phenotypically immature IgG-bearing B cells in patients with hypogammaglobulinemia', Journal of Clinical Immunology, vol. 6, no. 1, pp. 21-25. https://doi.org/10.1007/BF00915360
Fiorilli, Massimo ; Crescenzi, Marco ; Carbonari, Maurizio ; Tedesco, Letizia ; Russo, Giandomenico ; Gaetano, Carlo ; Aiuti, Fernando. / Phenotypically immature IgG-bearing B cells in patients with hypogammaglobulinemia. In: Journal of Clinical Immunology. 1986 ; Vol. 6, No. 1. pp. 21-25.
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