Abstract
Several distinct classes of drugs, such as anticonvulsants, immunosuppressants, and calcium channel blockers, caused gingival overgrowth. One of the main drugs associated with the gingival overgrowth is the anti-epileptic such as phenytoin, which affects gingival tissues by altering extracellular matrix metabolism. In our study, we evaluate the effect of phenytoin, a drug whose active substance is phenytoin, on gingival fibroblasts of healthy volunteers. Gene expression of 29 genes was investigated in gingival fibroblasts' cell culture treated with phenytoin compared with untreated cells. Among the studied genes, only 13 genes (CXCL5, CXCL10, CCR1, CCR3, CCR5, CCR6, IL-1A, IL-1B, IL-5, IL-7, IL-6R, BMP-2, and TNFSF-10) were statistically significant. All but one gene resulted downregulated after 24 h of treatment with phenytoin. BPM2 was the only, although weakly, up-expressed gene. Probably, we have not highlighted overexpression of the other inflammatory molecules because the study was performed on healthy people. Many studies show that phenytoin induces the overexpression of these cytokines but, probably, in our study, the drug does not have the same effect because we used gingival fibroblasts of healthy people.
| Original language | English |
|---|---|
| Article number | 2058738419828259 |
| Journal | International Journal of Immunopathology and Pharmacology |
| Volume | 33 |
| DOIs | |
| Publication status | Published - Jan 1 2019 |
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Keywords
- anti-epileptic drug (AED)
- gene expression
- gingival hyperplasia
- phenytoin
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Pharmacology
Cite this
Phenytoin and gingival mucosa : A molecular investigation. / Candotto, Valentina; Pezzetti, Furio; Baj, Alessandro; Beltramini, Giada; Lauritano, Dorina; Di Girolamo, Michele; Cura, Francesca.
In: International Journal of Immunopathology and Pharmacology, Vol. 33, 2058738419828259, 01.01.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Phenytoin and gingival mucosa
T2 - A molecular investigation
AU - Candotto, Valentina
AU - Pezzetti, Furio
AU - Baj, Alessandro
AU - Beltramini, Giada
AU - Lauritano, Dorina
AU - Di Girolamo, Michele
AU - Cura, Francesca
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Several distinct classes of drugs, such as anticonvulsants, immunosuppressants, and calcium channel blockers, caused gingival overgrowth. One of the main drugs associated with the gingival overgrowth is the anti-epileptic such as phenytoin, which affects gingival tissues by altering extracellular matrix metabolism. In our study, we evaluate the effect of phenytoin, a drug whose active substance is phenytoin, on gingival fibroblasts of healthy volunteers. Gene expression of 29 genes was investigated in gingival fibroblasts' cell culture treated with phenytoin compared with untreated cells. Among the studied genes, only 13 genes (CXCL5, CXCL10, CCR1, CCR3, CCR5, CCR6, IL-1A, IL-1B, IL-5, IL-7, IL-6R, BMP-2, and TNFSF-10) were statistically significant. All but one gene resulted downregulated after 24 h of treatment with phenytoin. BPM2 was the only, although weakly, up-expressed gene. Probably, we have not highlighted overexpression of the other inflammatory molecules because the study was performed on healthy people. Many studies show that phenytoin induces the overexpression of these cytokines but, probably, in our study, the drug does not have the same effect because we used gingival fibroblasts of healthy people.
AB - Several distinct classes of drugs, such as anticonvulsants, immunosuppressants, and calcium channel blockers, caused gingival overgrowth. One of the main drugs associated with the gingival overgrowth is the anti-epileptic such as phenytoin, which affects gingival tissues by altering extracellular matrix metabolism. In our study, we evaluate the effect of phenytoin, a drug whose active substance is phenytoin, on gingival fibroblasts of healthy volunteers. Gene expression of 29 genes was investigated in gingival fibroblasts' cell culture treated with phenytoin compared with untreated cells. Among the studied genes, only 13 genes (CXCL5, CXCL10, CCR1, CCR3, CCR5, CCR6, IL-1A, IL-1B, IL-5, IL-7, IL-6R, BMP-2, and TNFSF-10) were statistically significant. All but one gene resulted downregulated after 24 h of treatment with phenytoin. BPM2 was the only, although weakly, up-expressed gene. Probably, we have not highlighted overexpression of the other inflammatory molecules because the study was performed on healthy people. Many studies show that phenytoin induces the overexpression of these cytokines but, probably, in our study, the drug does not have the same effect because we used gingival fibroblasts of healthy people.
KW - anti-epileptic drug (AED)
KW - gene expression
KW - gingival hyperplasia
KW - phenytoin
UR - http://www.scopus.com/inward/record.url?scp=85074279747&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85074279747&partnerID=8YFLogxK
U2 - 10.1177/2058738419828259
DO - 10.1177/2058738419828259
M3 - Article
C2 - 31663446
AN - SCOPUS:85074279747
VL - 33
JO - International Journal of Immunopathology and Pharmacology
JF - International Journal of Immunopathology and Pharmacology
SN - 0394-6320
M1 - 2058738419828259
ER -