Phorbol ester induces abnormal chronic lymphocytic leukemia cells to express features of hairy cell leukemia

F. Caligaris-Cappio, G. Pizzolo, M. Chilosi, L. Bergui, G. Semenzato, L. Tesio, L. Morittu, F. Malavasi, M. Gobbi, R. Schwarting

Research output: Contribution to journalArticle

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Abstract

We have investigated the relationship between chronic lymphocytic leukemia (CLL), hair cell leukemia (HCL), and different normal B cell subsets: Mrbc+, T1+ and slgM+ tonsil cells; germinal center; mantle zone; and peripheral blood B lymphocytes. Both malignant and normal cells were incubated in vitro with the phorbol ester 12-O-tetradecanoyl-phorbol-13-acetate (TPA) for 72 hours and the morphology, cytochemical profile, and surface markers were evaluated. The results show that CLL cells TPA-induced become indistinguishable from HCL by four independent criteria: the morphology; the cytoplasmic tartrate-resistant acid phosphatase (TRAP) enzyme activity; the membrane positivity with anti-Leu M5 (SHCL3); and anti-Tac monoclonal antibodies which, in the uninduced state, react only with HCL. The features of TRAP and Tac positivity are also expressed (though in variable degree) by different normal B cell populations activated with TPA or pokeweed mitogen (PWM). It is concluded that HCL might represent an aberrantly activated variant of CLL (or of a CLL-related disorder).

Original languageEnglish
Pages (from-to)1035-1042
Number of pages8
JournalBlood
Volume66
Issue number5
Publication statusPublished - 1985

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Hairy Cell Leukemia
Phorbol Esters
Tetradecanoylphorbol Acetate
B-Cell Chronic Lymphocytic Leukemia
Cells
Acid Phosphatase
Leukemia
Pokeweed Mitogens
Lymphocytes
Enzyme activity
Blood
Monoclonal Antibodies
B-Lymphocytes
Membranes
B-Lymphocyte Subsets
Germinal Center
Palatine Tonsil
tartaric acid
Enzymes
Population

ASJC Scopus subject areas

  • Hematology

Cite this

Caligaris-Cappio, F., Pizzolo, G., Chilosi, M., Bergui, L., Semenzato, G., Tesio, L., ... Schwarting, R. (1985). Phorbol ester induces abnormal chronic lymphocytic leukemia cells to express features of hairy cell leukemia. Blood, 66(5), 1035-1042.

Phorbol ester induces abnormal chronic lymphocytic leukemia cells to express features of hairy cell leukemia. / Caligaris-Cappio, F.; Pizzolo, G.; Chilosi, M.; Bergui, L.; Semenzato, G.; Tesio, L.; Morittu, L.; Malavasi, F.; Gobbi, M.; Schwarting, R.

In: Blood, Vol. 66, No. 5, 1985, p. 1035-1042.

Research output: Contribution to journalArticle

Caligaris-Cappio, F, Pizzolo, G, Chilosi, M, Bergui, L, Semenzato, G, Tesio, L, Morittu, L, Malavasi, F, Gobbi, M & Schwarting, R 1985, 'Phorbol ester induces abnormal chronic lymphocytic leukemia cells to express features of hairy cell leukemia', Blood, vol. 66, no. 5, pp. 1035-1042.
Caligaris-Cappio F, Pizzolo G, Chilosi M, Bergui L, Semenzato G, Tesio L et al. Phorbol ester induces abnormal chronic lymphocytic leukemia cells to express features of hairy cell leukemia. Blood. 1985;66(5):1035-1042.
Caligaris-Cappio, F. ; Pizzolo, G. ; Chilosi, M. ; Bergui, L. ; Semenzato, G. ; Tesio, L. ; Morittu, L. ; Malavasi, F. ; Gobbi, M. ; Schwarting, R. / Phorbol ester induces abnormal chronic lymphocytic leukemia cells to express features of hairy cell leukemia. In: Blood. 1985 ; Vol. 66, No. 5. pp. 1035-1042.
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AU - Pizzolo, G.

AU - Chilosi, M.

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AU - Semenzato, G.

AU - Tesio, L.

AU - Morittu, L.

AU - Malavasi, F.

AU - Gobbi, M.

AU - Schwarting, R.

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AB - We have investigated the relationship between chronic lymphocytic leukemia (CLL), hair cell leukemia (HCL), and different normal B cell subsets: Mrbc+, T1+ and slgM+ tonsil cells; germinal center; mantle zone; and peripheral blood B lymphocytes. Both malignant and normal cells were incubated in vitro with the phorbol ester 12-O-tetradecanoyl-phorbol-13-acetate (TPA) for 72 hours and the morphology, cytochemical profile, and surface markers were evaluated. The results show that CLL cells TPA-induced become indistinguishable from HCL by four independent criteria: the morphology; the cytoplasmic tartrate-resistant acid phosphatase (TRAP) enzyme activity; the membrane positivity with anti-Leu M5 (SHCL3); and anti-Tac monoclonal antibodies which, in the uninduced state, react only with HCL. The features of TRAP and Tac positivity are also expressed (though in variable degree) by different normal B cell populations activated with TPA or pokeweed mitogen (PWM). It is concluded that HCL might represent an aberrantly activated variant of CLL (or of a CLL-related disorder).

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