Phosphatidylinositol 3-kinase is recruited to a specific site in the activated IL-1 receptor I

Sandra Marmiroli, Alberto Bavelloni, Irene Faenza, Alessandra Sirri, Andrea Ognibene, Vittoria Cenni, Junichi Tsukada, Yoshinobu Koyama, Maria Ruzzene, Alberto Ferri, Philip E. Auron, Alex Toker, Nadir M. Maraldi

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Interleukin 1 (IL-1) delivers a stimulatory signal which increases the expression of a set of genes by modulating the transcription factor NF-κB. The IL-1 receptors are transmembrane glycoproteins which lack a catalytic domain. The C-terminal portion of the type I IL-1 receptor (IL-1RI) is essential for IL-1 signalling and for IL-1 dependent activation of NF-κB. This portion contains a putative phosphatidylinositol 3-kinase (PI 3-kinase) binding domain (Tyr-E-X-Met), which is highly conserved between the human, mouse and chicken sequences, as well as the related cytoplasmic domain of the Drosophila receptor Toll. This observation prompted us to investigate the role of PI 3-kinase in IL-1 signalling. Here we report evidence that PI 3-kinase is recruited by the activated IL-1RI, causing rapid and transient activation of PI 3-kinase. We also show that the receptor is tyrosine phosphorylated in response to IL-1. Expression of a receptor mutant lacking the putative binding site for p85 demonstrates that Tyr 479 in the receptor cytoplasmic domain is essential for PI 3-kinase activation by IL-1. Our results indicate that PI 3-kinase is likely to be an important mediator of some IL-1 effects, providing docking sites for additional signalling molecules. Copyright (C) 1998 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)49-54
Number of pages6
JournalFEBS Letters
Volume438
Issue number1-2
DOIs
Publication statusPublished - Oct 30 1998

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Phosphatidylinositol 3-Kinase
Interleukin-1 Receptors
Interleukin-1
Chemical activation
Interleukin-1 Type I Receptors
Cytoplasmic and Nuclear Receptors
Drosophila
Tyrosine
Chickens
Catalytic Domain
Glycoproteins
Transcription Factors
Genes
Binding Sites
Molecules

Keywords

  • Human osteosarcoma cell line
  • Interleukin-1 receptor
  • Nuclear factor κB
  • Phosphatidylinositol 3-kinase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Marmiroli, S., Bavelloni, A., Faenza, I., Sirri, A., Ognibene, A., Cenni, V., ... Maraldi, N. M. (1998). Phosphatidylinositol 3-kinase is recruited to a specific site in the activated IL-1 receptor I. FEBS Letters, 438(1-2), 49-54. https://doi.org/10.1016/S0014-5793(98)01270-8

Phosphatidylinositol 3-kinase is recruited to a specific site in the activated IL-1 receptor I. / Marmiroli, Sandra; Bavelloni, Alberto; Faenza, Irene; Sirri, Alessandra; Ognibene, Andrea; Cenni, Vittoria; Tsukada, Junichi; Koyama, Yoshinobu; Ruzzene, Maria; Ferri, Alberto; Auron, Philip E.; Toker, Alex; Maraldi, Nadir M.

In: FEBS Letters, Vol. 438, No. 1-2, 30.10.1998, p. 49-54.

Research output: Contribution to journalArticle

Marmiroli, S, Bavelloni, A, Faenza, I, Sirri, A, Ognibene, A, Cenni, V, Tsukada, J, Koyama, Y, Ruzzene, M, Ferri, A, Auron, PE, Toker, A & Maraldi, NM 1998, 'Phosphatidylinositol 3-kinase is recruited to a specific site in the activated IL-1 receptor I', FEBS Letters, vol. 438, no. 1-2, pp. 49-54. https://doi.org/10.1016/S0014-5793(98)01270-8
Marmiroli, Sandra ; Bavelloni, Alberto ; Faenza, Irene ; Sirri, Alessandra ; Ognibene, Andrea ; Cenni, Vittoria ; Tsukada, Junichi ; Koyama, Yoshinobu ; Ruzzene, Maria ; Ferri, Alberto ; Auron, Philip E. ; Toker, Alex ; Maraldi, Nadir M. / Phosphatidylinositol 3-kinase is recruited to a specific site in the activated IL-1 receptor I. In: FEBS Letters. 1998 ; Vol. 438, No. 1-2. pp. 49-54.
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