Phosphatidylinositol 4-phosphate 5-kinase β controls recruitment of lipid rafts into the immunological synapse

Marinos Kallikourdis, Anna Elisa Trovato, Giuliana Roselli, Michela Muscolini, Nicla Porciello, Loretta Tuosto, Antonella Viola

Research output: Contribution to journalArticle

Abstract

Phosphatidylinositol 4,5-biphosphate (PIP2) is critical for T lymphocyte activation serving as a substrate for the generation of second messengers and the remodeling of actin cytoskeleton necessary for the clustering of lipid rafts, TCR, and costimulatory receptors toward the T:APC interface. Spatiotemporal analysis of PIP2 synthesis in T lymphocytes suggested that distinct isoforms of the main PIP2-generating enzyme, phosphatidylinositol 4-phosphate 5-kinase (PIP5K), play a differential role on the basis of their distinct localization. In this study, we analyze the contribution of PIP5Kb to T cell activation and show that CD28 induces the recruitment of PIP5Kb to the immunological synapse, where it regulates filamin A and lipid raft accumulation, as well as T cell activation, in a nonredundant manner. Finally, we found that Vav1 and the C-terminal 83 aa of PIP5Kb are pivotal for the PIP5Kb regulatory functions in response to CD28 stimulation.

Original languageEnglish
Pages (from-to)1955-1963
Number of pages9
JournalJournal of Immunology
Volume196
Issue number4
DOIs
Publication statusPublished - Feb 15 2016

ASJC Scopus subject areas

  • Immunology

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