TY - JOUR
T1 - Phosphatidylinositol turnover is not a general regulator of neuroblastoma cell differentiation
T2 - comparison between two differentiating agents, retinoic acid and γ-interferon
AU - Lanciotti, M.
AU - Cornaglia-Ferraris, P.
AU - Ponzoni, M.
PY - 1989/1/30
Y1 - 1989/1/30
N2 - The turnover of phosphatidylinositol (PI) is believed to constitute a crucial step in the signaling pathways for stimulation of cells by a variety of bioactive substances, including differentiating agents; however decisive evidence for the idea has not been obtained. In the present paper, we investigated the involvement of PI turnover in cell differentiation using a human neuroblastoma cell line, LAN-1, which can be induced to differentiate along the neuronal pathway by both retinoic acid (RA) and γ-interferon (γ-IFN). Analysis of labelled phosphatidylinositol metabolises from prelabelled cells indicated a rapid decrease of inositol 1,4,5-trisphosphate and 1,2-diacylglycerol within 1 min of induction of LAN-1 cell dilfferentiation by RA, while no changes were observed in γ-IFN-treated cells. These findings indicate the occurrence of decreased inositol phospholipid turnover in RA-treated LAN-1 cells and suggest that phosphoinositide-derived metabolises may not constitute general regulators of cellular differentiation.
AB - The turnover of phosphatidylinositol (PI) is believed to constitute a crucial step in the signaling pathways for stimulation of cells by a variety of bioactive substances, including differentiating agents; however decisive evidence for the idea has not been obtained. In the present paper, we investigated the involvement of PI turnover in cell differentiation using a human neuroblastoma cell line, LAN-1, which can be induced to differentiate along the neuronal pathway by both retinoic acid (RA) and γ-interferon (γ-IFN). Analysis of labelled phosphatidylinositol metabolises from prelabelled cells indicated a rapid decrease of inositol 1,4,5-trisphosphate and 1,2-diacylglycerol within 1 min of induction of LAN-1 cell dilfferentiation by RA, while no changes were observed in γ-IFN-treated cells. These findings indicate the occurrence of decreased inositol phospholipid turnover in RA-treated LAN-1 cells and suggest that phosphoinositide-derived metabolises may not constitute general regulators of cellular differentiation.
KW - Differentiation
KW - Interferon-γ
KW - Phosphatidylinositol turnover
KW - Retinoic acid
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U2 - 10.1016/0014-5793(89)80146-2
DO - 10.1016/0014-5793(89)80146-2
M3 - Article
C2 - 2492954
AN - SCOPUS:0024557705
VL - 243
SP - 285
EP - 288
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 2
ER -