TY - JOUR
T1 - Phosphoantigen-reactive Vγ9Vδ2 T lymphocytes suppress in vitro human immunodeficiency virus type 1 replication by cell-released antiviral factors including CC chemokines
AU - Poccia, Fabrizio
AU - Battistini, Luca
AU - Cipriani, Barbara
AU - Mancino, Giorgio
AU - Martini, Federico
AU - Gougeon, Marie Lise
AU - Colizzi, Vittorio
PY - 1999
Y1 - 1999
N2 - Vγ9Vδ2 T lymphocytes are broadly reactive against various intracellular pathogens and display both lyric and proliferative responses to human immunodeficiency virus (HIV)-infected cells. HIV infection of peripheral blood mononuclear cell cultures led to absolute increases in Vγ9Vδ2 T cells accompanied by decreased p24 levels. Strong γδ T cell activation with nonpeptidic mycobacterial phosphoantigens (TUBAg1 extract or synthetic isopentenyl pyrophosphate) resulted in potent inhibition of HIV replication through soluble released factors. Subsequent analyses showed that phosphoantigen-activated γδ T cells produced substantial amounts of β- chemokines (macrophage inflammatory protein [MIP]-1α, MIP-1β, and regulated-on-activation, normal T-cell-expressed and -secreted beta-chemokine [RANTES]), which represent the natural ligand for the CCR5 HIV coreceptor. Accordingly, anti-β-chemokine antibodies neutralized the inhibition of monocytotropic HIV strains by γδ T cell-released factors. Moreover, a T- tropic HIV strain using the CXCR4 coreceptor for virus entry was potently inhibited. Together, these data reveal that phosphoantigen-activated γδ T cells are an important source of CC chemokines and may suppress HIV replication through cell-released antiviral factors.
AB - Vγ9Vδ2 T lymphocytes are broadly reactive against various intracellular pathogens and display both lyric and proliferative responses to human immunodeficiency virus (HIV)-infected cells. HIV infection of peripheral blood mononuclear cell cultures led to absolute increases in Vγ9Vδ2 T cells accompanied by decreased p24 levels. Strong γδ T cell activation with nonpeptidic mycobacterial phosphoantigens (TUBAg1 extract or synthetic isopentenyl pyrophosphate) resulted in potent inhibition of HIV replication through soluble released factors. Subsequent analyses showed that phosphoantigen-activated γδ T cells produced substantial amounts of β- chemokines (macrophage inflammatory protein [MIP]-1α, MIP-1β, and regulated-on-activation, normal T-cell-expressed and -secreted beta-chemokine [RANTES]), which represent the natural ligand for the CCR5 HIV coreceptor. Accordingly, anti-β-chemokine antibodies neutralized the inhibition of monocytotropic HIV strains by γδ T cell-released factors. Moreover, a T- tropic HIV strain using the CXCR4 coreceptor for virus entry was potently inhibited. Together, these data reveal that phosphoantigen-activated γδ T cells are an important source of CC chemokines and may suppress HIV replication through cell-released antiviral factors.
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U2 - 10.1086/314925
DO - 10.1086/314925
M3 - Article
C2 - 10438380
AN - SCOPUS:0033190539
VL - 180
SP - 858
EP - 861
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 3
ER -