Phosphoinositidase C isozymes in SaOS-2 cells: Immunocytochemical detection in nuclear and cytoplasmic compartments

Nadir M. Maraldi, Nicoletta Zini, Spartaco Santi, Alberto Bavelloni, Aurelio Valmori, Sandra Marmiroli, Andrea Ognibene

Research output: Contribution to journalArticle

Abstract

SaOS-2 cell line presents osteoblastic characteristics which can be modulated by specific agonists involving also phosphoinositide breakdown. In order to determine whether SaOS-2 cells display a phosphoinositide signalling system not only at the cytosol-cell membrane level but also, as recently reported for other cell lines, at the nuclear level, a study has been performed to evaluate the phosphoinositidase C (PIC) activity and to localize different isoforms of PIC in nuclear and cytoplasmic compartments. By immunochemicals methods, and by confocal and electron microscope immunocytochemistry, both PIC β1 and γ1 have been detected in the nucleus, while only PIC γ1 was found in the cytoplasm. A specific association with the inner nuclear matrix has been demonstrated for PIC β1 and γ1; this latter resulted, on the other hand, in relationship with cytoskeletal filaments after high salt extraction. These findings suggest that these enzymes are not completely soluble but functionally related with cytoskeletal and nucleoskeletal structures.

Original languageEnglish
Pages (from-to)243-250
Number of pages8
JournalBiology of the cell / under the auspices of the European Cell Biology Organization
Volume79
Issue number3
Publication statusPublished - 1993

ASJC Scopus subject areas

  • Cell Biology

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