Phosphoinositide-derived diacylglycerol conversion to phosphatidic acid is a receptor-dependent and compartmentalized phenomenon in human neuroblastoma

Cristina Limatola, Laura Pacini, Irene Ricci, Angelo Spinedi

Research output: Contribution to journalArticlepeer-review

Abstract

We report that upon muscarinic stimulation of SI(-N-BE(2) human neuroblastoma cells, the extent of phosphoinositide-derived diacylglycerol (DG) conversion to phosphatidic acid (PA), operated by a DG kinase, is dependent on the potency of receptor stimulation and correlates with the reduction of phosphatidylinositol 4,5-bisphosphate mass. Evidence is provided that agonist-evoked Ca 2+ mobilisation or protein kinase activation are not key events in triggering receptor-generated DG conversion to PA; furthermore, the phenomenon is compartmentalized, namely it occurs within a topologically restricted area that is poorly accessible to DG artificially generated by cell treatment with bacterial phosphatidylinositol-specific phospholipase C. Possible mechanisms driving regulation of the DG kinase operating in the transduction system investigated are discussed.

Original languageEnglish
Pages (from-to)127-130
Number of pages4
JournalNeuroscience Letters
Volume219
Issue number2
DOIs
Publication statusPublished - Nov 22 1996

Keywords

  • Carbachol
  • Diacylglycerol
  • Diacylglycerol kinase
  • Neuroblastoma
  • Phosphatidic acid

ASJC Scopus subject areas

  • Neuroscience(all)

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