TY - JOUR
T1 - Phosphoinositide signaling in nuclei of friend cells
T2 - Tiazofurin down-regulates phospholipase C β1
AU - Manzoli, Lucia
AU - Billi, Anna Maria
AU - Gilmour, R. Stewart
AU - Martelli, Alberto Maria
AU - Matteucci, Alessandro
AU - Rubbini, Silvia
AU - Weber, George
AU - Cocco, Lucio
PY - 1995
Y1 - 1995
N2 - Previous investigations have demonstrated the presence of conventional lipid kinases and phospholipase C (PLC) activities in nuclei of Friend erythroleukemia cells. Moreover, when Friend erythroleukemia cells are treated for 96 h with the antitumor drug tiazofurin, the induction of erythroid differentiation is accompanied by changes in amounts of both phosphatidylinositol and phosphatidylinositol 4,5-bisphosphate due to the inhibition of an uncharacterized nuclear PLC activity. Here, we show that the nuclear PLC β1 isoform is down-regulated by tiazofurin (5 μM) treatment of Friend erythroleukemia cells as shown by both Western blot and Northern blot analyses for PLC β1 message. This indicates that PLC β1 down-regulation is tightly linked with erythroid differentiation of Friend erythroleukemia cells and that the autonomous nuclear signaling via inositol lipid cycle can be controlled by the antitumor drug tiazofurin.
AB - Previous investigations have demonstrated the presence of conventional lipid kinases and phospholipase C (PLC) activities in nuclei of Friend erythroleukemia cells. Moreover, when Friend erythroleukemia cells are treated for 96 h with the antitumor drug tiazofurin, the induction of erythroid differentiation is accompanied by changes in amounts of both phosphatidylinositol and phosphatidylinositol 4,5-bisphosphate due to the inhibition of an uncharacterized nuclear PLC activity. Here, we show that the nuclear PLC β1 isoform is down-regulated by tiazofurin (5 μM) treatment of Friend erythroleukemia cells as shown by both Western blot and Northern blot analyses for PLC β1 message. This indicates that PLC β1 down-regulation is tightly linked with erythroid differentiation of Friend erythroleukemia cells and that the autonomous nuclear signaling via inositol lipid cycle can be controlled by the antitumor drug tiazofurin.
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M3 - Article
C2 - 7606713
AN - SCOPUS:0029072097
VL - 55
SP - 2978
EP - 2980
JO - Journal of Cancer Research
JF - Journal of Cancer Research
SN - 0008-5472
IS - 14
ER -