Phosphoinositide signaling in nuclei of friend cells: Tiazofurin down-regulates phospholipase C β1

Lucia Manzoli, Anna Maria Billi, R. Stewart Gilmour, Alberto Maria Martelli, Alessandro Matteucci, Silvia Rubbini, George Weber, Lucio Cocco

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Previous investigations have demonstrated the presence of conventional lipid kinases and phospholipase C (PLC) activities in nuclei of Friend erythroleukemia cells. Moreover, when Friend erythroleukemia cells are treated for 96 h with the antitumor drug tiazofurin, the induction of erythroid differentiation is accompanied by changes in amounts of both phosphatidylinositol and phosphatidylinositol 4,5-bisphosphate due to the inhibition of an uncharacterized nuclear PLC activity. Here, we show that the nuclear PLC β1 isoform is down-regulated by tiazofurin (5 μM) treatment of Friend erythroleukemia cells as shown by both Western blot and Northern blot analyses for PLC β1 message. This indicates that PLC β1 down-regulation is tightly linked with erythroid differentiation of Friend erythroleukemia cells and that the autonomous nuclear signaling via inositol lipid cycle can be controlled by the antitumor drug tiazofurin.

Original languageEnglish
Pages (from-to)2978-2980
Number of pages3
JournalCancer Research
Volume55
Issue number14
Publication statusPublished - 1995

Fingerprint

tiazofurin
Type C Phospholipases
Phosphatidylinositols
Cell Nucleus
Leukemia, Erythroblastic, Acute
Down-Regulation
Antineoplastic Agents
Lipids
Inositol
Northern Blotting
Protein Isoforms
Phosphotransferases
Western Blotting

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Manzoli, L., Billi, A. M., Gilmour, R. S., Martelli, A. M., Matteucci, A., Rubbini, S., ... Cocco, L. (1995). Phosphoinositide signaling in nuclei of friend cells: Tiazofurin down-regulates phospholipase C β1. Cancer Research, 55(14), 2978-2980.

Phosphoinositide signaling in nuclei of friend cells : Tiazofurin down-regulates phospholipase C β1. / Manzoli, Lucia; Billi, Anna Maria; Gilmour, R. Stewart; Martelli, Alberto Maria; Matteucci, Alessandro; Rubbini, Silvia; Weber, George; Cocco, Lucio.

In: Cancer Research, Vol. 55, No. 14, 1995, p. 2978-2980.

Research output: Contribution to journalArticle

Manzoli, L, Billi, AM, Gilmour, RS, Martelli, AM, Matteucci, A, Rubbini, S, Weber, G & Cocco, L 1995, 'Phosphoinositide signaling in nuclei of friend cells: Tiazofurin down-regulates phospholipase C β1', Cancer Research, vol. 55, no. 14, pp. 2978-2980.
Manzoli L, Billi AM, Gilmour RS, Martelli AM, Matteucci A, Rubbini S et al. Phosphoinositide signaling in nuclei of friend cells: Tiazofurin down-regulates phospholipase C β1. Cancer Research. 1995;55(14):2978-2980.
Manzoli, Lucia ; Billi, Anna Maria ; Gilmour, R. Stewart ; Martelli, Alberto Maria ; Matteucci, Alessandro ; Rubbini, Silvia ; Weber, George ; Cocco, Lucio. / Phosphoinositide signaling in nuclei of friend cells : Tiazofurin down-regulates phospholipase C β1. In: Cancer Research. 1995 ; Vol. 55, No. 14. pp. 2978-2980.
@article{f68b92d5e543461eb5dcb9b41ab3173e,
title = "Phosphoinositide signaling in nuclei of friend cells: Tiazofurin down-regulates phospholipase C β1",
abstract = "Previous investigations have demonstrated the presence of conventional lipid kinases and phospholipase C (PLC) activities in nuclei of Friend erythroleukemia cells. Moreover, when Friend erythroleukemia cells are treated for 96 h with the antitumor drug tiazofurin, the induction of erythroid differentiation is accompanied by changes in amounts of both phosphatidylinositol and phosphatidylinositol 4,5-bisphosphate due to the inhibition of an uncharacterized nuclear PLC activity. Here, we show that the nuclear PLC β1 isoform is down-regulated by tiazofurin (5 μM) treatment of Friend erythroleukemia cells as shown by both Western blot and Northern blot analyses for PLC β1 message. This indicates that PLC β1 down-regulation is tightly linked with erythroid differentiation of Friend erythroleukemia cells and that the autonomous nuclear signaling via inositol lipid cycle can be controlled by the antitumor drug tiazofurin.",
author = "Lucia Manzoli and Billi, {Anna Maria} and Gilmour, {R. Stewart} and Martelli, {Alberto Maria} and Alessandro Matteucci and Silvia Rubbini and George Weber and Lucio Cocco",
year = "1995",
language = "English",
volume = "55",
pages = "2978--2980",
journal = "Journal of Cancer Research",
issn = "0008-5472",
publisher = "American Association for Cancer Research Inc.",
number = "14",

}

TY - JOUR

T1 - Phosphoinositide signaling in nuclei of friend cells

T2 - Tiazofurin down-regulates phospholipase C β1

AU - Manzoli, Lucia

AU - Billi, Anna Maria

AU - Gilmour, R. Stewart

AU - Martelli, Alberto Maria

AU - Matteucci, Alessandro

AU - Rubbini, Silvia

AU - Weber, George

AU - Cocco, Lucio

PY - 1995

Y1 - 1995

N2 - Previous investigations have demonstrated the presence of conventional lipid kinases and phospholipase C (PLC) activities in nuclei of Friend erythroleukemia cells. Moreover, when Friend erythroleukemia cells are treated for 96 h with the antitumor drug tiazofurin, the induction of erythroid differentiation is accompanied by changes in amounts of both phosphatidylinositol and phosphatidylinositol 4,5-bisphosphate due to the inhibition of an uncharacterized nuclear PLC activity. Here, we show that the nuclear PLC β1 isoform is down-regulated by tiazofurin (5 μM) treatment of Friend erythroleukemia cells as shown by both Western blot and Northern blot analyses for PLC β1 message. This indicates that PLC β1 down-regulation is tightly linked with erythroid differentiation of Friend erythroleukemia cells and that the autonomous nuclear signaling via inositol lipid cycle can be controlled by the antitumor drug tiazofurin.

AB - Previous investigations have demonstrated the presence of conventional lipid kinases and phospholipase C (PLC) activities in nuclei of Friend erythroleukemia cells. Moreover, when Friend erythroleukemia cells are treated for 96 h with the antitumor drug tiazofurin, the induction of erythroid differentiation is accompanied by changes in amounts of both phosphatidylinositol and phosphatidylinositol 4,5-bisphosphate due to the inhibition of an uncharacterized nuclear PLC activity. Here, we show that the nuclear PLC β1 isoform is down-regulated by tiazofurin (5 μM) treatment of Friend erythroleukemia cells as shown by both Western blot and Northern blot analyses for PLC β1 message. This indicates that PLC β1 down-regulation is tightly linked with erythroid differentiation of Friend erythroleukemia cells and that the autonomous nuclear signaling via inositol lipid cycle can be controlled by the antitumor drug tiazofurin.

UR - http://www.scopus.com/inward/record.url?scp=0029072097&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029072097&partnerID=8YFLogxK

M3 - Article

C2 - 7606713

AN - SCOPUS:0029072097

VL - 55

SP - 2978

EP - 2980

JO - Journal of Cancer Research

JF - Journal of Cancer Research

SN - 0008-5472

IS - 14

ER -