Abstract
Previous investigations have demonstrated the presence of conventional lipid kinases and phospholipase C (PLC) activities in nuclei of Friend erythroleukemia cells. Moreover, when Friend erythroleukemia cells are treated for 96 h with the antitumor drug tiazofurin, the induction of erythroid differentiation is accompanied by changes in amounts of both phosphatidylinositol and phosphatidylinositol 4,5-bisphosphate due to the inhibition of an uncharacterized nuclear PLC activity. Here, we show that the nuclear PLC β1 isoform is down-regulated by tiazofurin (5 μM) treatment of Friend erythroleukemia cells as shown by both Western blot and Northern blot analyses for PLC β1 message. This indicates that PLC β1 down-regulation is tightly linked with erythroid differentiation of Friend erythroleukemia cells and that the autonomous nuclear signaling via inositol lipid cycle can be controlled by the antitumor drug tiazofurin.
| Original language | English |
|---|---|
| Pages (from-to) | 2978-2980 |
| Number of pages | 3 |
| Journal | Cancer Research |
| Volume | 55 |
| Issue number | 14 |
| Publication status | Published - 1995 |
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ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Phosphoinositide signaling in nuclei of friend cells : Tiazofurin down-regulates phospholipase C β1. / Manzoli, Lucia; Billi, Anna Maria; Gilmour, R. Stewart; Martelli, Alberto Maria; Matteucci, Alessandro; Rubbini, Silvia; Weber, George; Cocco, Lucio.
In: Cancer Research, Vol. 55, No. 14, 1995, p. 2978-2980.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Phosphoinositide signaling in nuclei of friend cells
T2 - Tiazofurin down-regulates phospholipase C β1
AU - Manzoli, Lucia
AU - Billi, Anna Maria
AU - Gilmour, R. Stewart
AU - Martelli, Alberto Maria
AU - Matteucci, Alessandro
AU - Rubbini, Silvia
AU - Weber, George
AU - Cocco, Lucio
PY - 1995
Y1 - 1995
N2 - Previous investigations have demonstrated the presence of conventional lipid kinases and phospholipase C (PLC) activities in nuclei of Friend erythroleukemia cells. Moreover, when Friend erythroleukemia cells are treated for 96 h with the antitumor drug tiazofurin, the induction of erythroid differentiation is accompanied by changes in amounts of both phosphatidylinositol and phosphatidylinositol 4,5-bisphosphate due to the inhibition of an uncharacterized nuclear PLC activity. Here, we show that the nuclear PLC β1 isoform is down-regulated by tiazofurin (5 μM) treatment of Friend erythroleukemia cells as shown by both Western blot and Northern blot analyses for PLC β1 message. This indicates that PLC β1 down-regulation is tightly linked with erythroid differentiation of Friend erythroleukemia cells and that the autonomous nuclear signaling via inositol lipid cycle can be controlled by the antitumor drug tiazofurin.
AB - Previous investigations have demonstrated the presence of conventional lipid kinases and phospholipase C (PLC) activities in nuclei of Friend erythroleukemia cells. Moreover, when Friend erythroleukemia cells are treated for 96 h with the antitumor drug tiazofurin, the induction of erythroid differentiation is accompanied by changes in amounts of both phosphatidylinositol and phosphatidylinositol 4,5-bisphosphate due to the inhibition of an uncharacterized nuclear PLC activity. Here, we show that the nuclear PLC β1 isoform is down-regulated by tiazofurin (5 μM) treatment of Friend erythroleukemia cells as shown by both Western blot and Northern blot analyses for PLC β1 message. This indicates that PLC β1 down-regulation is tightly linked with erythroid differentiation of Friend erythroleukemia cells and that the autonomous nuclear signaling via inositol lipid cycle can be controlled by the antitumor drug tiazofurin.
UR - http://www.scopus.com/inward/record.url?scp=0029072097&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029072097&partnerID=8YFLogxK
M3 - Article
VL - 55
SP - 2978
EP - 2980
JO - Journal of Cancer Research
JF - Journal of Cancer Research
SN - 0008-5472
IS - 14
ER -