Phospholipase A2 activity and calpactin I levels in rat lymphokine-activated killer cells

Correlation with the cytotoxic activity

M. Grazia Cifone, Luisa Cironi, Paola Roncaioli, Stefano Martinotti, Elena Toniato, Lucia Cilenti, Dario Botti, Egle Solito, Luca Parente, Angela Santoni

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In the present paper we have shown evidence for a significant increase of type II sPLA2 activity in A-LAK cells. The A-LAX-mediated cytotoxicity against YAC-1 target cells was strongly inhibited by two inhibitors of sPLA2, p-BPB and mepacrine, suggesting the involvement of this enzyme in the lytic mechanism of A-LAK. On the other hand, stimuli such as A23187 ionophore and TPA, which were able to induce in control cells an increased AA release, failed to cause this effect in IL-2-treated cells, suggesting that PLA2 was not active in these cells. Thus, we analyzed the levels of calpactin I, which is considered to be involved in the down-regulation of PLA2 activity. HrIL-2 treatment led to an increased expression of calpactin I at both the RNA and the protein level. A substantial portion of calpactin I was associated with the external surface of A-LAK and was able to exert a strong inhibitory effect on a purified porcine pancreatic PLA2 activity in vitro. Our results suggest that the role of calpactin I could be relevant to regulate PLA2 activity, and to protect the effector cells against a possible toxic effect which this enzyme could exert if present at high levels.

Original languageEnglish
Pages (from-to)274-282
Number of pages9
JournalCellular Immunology
Volume170
Issue number2
DOIs
Publication statusPublished - Jun 15 1996

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Annexin A2
Lymphokine-Activated Killer Cells
Phospholipases A2
Quinacrine
Poisons
Ionophores
Calcimycin
Enzymes
Interleukin-2
Swine
Down-Regulation
RNA
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Cifone, M. G., Cironi, L., Roncaioli, P., Martinotti, S., Toniato, E., Cilenti, L., ... Santoni, A. (1996). Phospholipase A2 activity and calpactin I levels in rat lymphokine-activated killer cells: Correlation with the cytotoxic activity. Cellular Immunology, 170(2), 274-282. https://doi.org/10.1006/cimm.1996.0161

Phospholipase A2 activity and calpactin I levels in rat lymphokine-activated killer cells : Correlation with the cytotoxic activity. / Cifone, M. Grazia; Cironi, Luisa; Roncaioli, Paola; Martinotti, Stefano; Toniato, Elena; Cilenti, Lucia; Botti, Dario; Solito, Egle; Parente, Luca; Santoni, Angela.

In: Cellular Immunology, Vol. 170, No. 2, 15.06.1996, p. 274-282.

Research output: Contribution to journalArticle

Cifone, MG, Cironi, L, Roncaioli, P, Martinotti, S, Toniato, E, Cilenti, L, Botti, D, Solito, E, Parente, L & Santoni, A 1996, 'Phospholipase A2 activity and calpactin I levels in rat lymphokine-activated killer cells: Correlation with the cytotoxic activity', Cellular Immunology, vol. 170, no. 2, pp. 274-282. https://doi.org/10.1006/cimm.1996.0161
Cifone, M. Grazia ; Cironi, Luisa ; Roncaioli, Paola ; Martinotti, Stefano ; Toniato, Elena ; Cilenti, Lucia ; Botti, Dario ; Solito, Egle ; Parente, Luca ; Santoni, Angela. / Phospholipase A2 activity and calpactin I levels in rat lymphokine-activated killer cells : Correlation with the cytotoxic activity. In: Cellular Immunology. 1996 ; Vol. 170, No. 2. pp. 274-282.
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