Phospholipase Cγ1 is required for metastasis development and progression

Gianluca Sala, Francesco Dituri, Claudio Raimondi, Sara Previdi, Tania Maffucci, Marco Mazzoletti, Cosmo Rossi, Manuela Iezzi, Rossano Lattanzio, Mauro Piantelli, Stefano Iacobelli, Massimo Broggini, Marco Falasca

Research output: Contribution to journalArticlepeer-review

Abstract

Cell motility and invasion play an essential role in the development of metastasis. Evidence suggests that the enzyme phospholipase Cγ1 (PLCγ1) may be involved in tumor progression and possibly development of metastasis. In this study, we show that down-regulation of PLCγ1 expression severely impairs activation of the small GTP-binding protein Rac and cell invasion in breast cancer cell lines and U87 in vitro. Experimental metastasis assays in nude mice show that inducible knockdown of PLCγ1 strongly inhibits development of MDA-MB-231-derived lung metastasis and reverts metastasis formation. In addition, analysis of 60 breast cancer patients' tissues revealed an increase of PLCγ1 expression in metastasis compared with the primary tumor in 50% of tissues analyzed. These data showa critical role of PLCγ1 in the metastatic potential of cancer cells, and they further indicate that PLCγ1 inhibition has a therapeutic potential in the treatment of metastasis dissemination.

Original languageEnglish
Pages (from-to)10187-10196
Number of pages10
JournalCancer Research
Volume68
Issue number24
DOIs
Publication statusPublished - Dec 15 2008

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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