Phosphorylase isoenzymes of human brain

Nereo Bresolin, Armand F. Miranda, Mercedes P. Jacobson, Jeen Hyung Lee, Thomas Capilupi, Salvatore Dimauro

Research output: Contribution to journalArticle

Abstract

The isoenzyme pattern of phosphorylase in human brain was studied by polyacrylamide slab gel electrophoresis and activity was revealed by iodine stain and by autoradiography: A major fast-migrating band was similar to isoenzyme I of the heart; an intermediate band migrated like the hybrid cardiac isoenzyme II; and a slow-migrating band, best revealed by autoradiography, migrated like the "muscle" isoenzyme III. The radioactivity in bands II and III accounted for 42% of total activity in the heart and 37% in the brain. Antibodies against mature muscle phosphorylase inhibited 41% of enzyme activity in heart extracts and 25% in brain extracts. These studies show that the "muscle" isoenzyme is a component of brain phosphorylase and suggest that partial phosphorylase deficiency occurs in the brain of patients with McArdle disease.

Original languageEnglish
Pages (from-to)171-178
Number of pages8
JournalNeurochemical Pathology
Volume1
Issue number3
DOIs
Publication statusPublished - Sep 1983

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Phosphorylases
Isoenzymes
Brain
Autoradiography
Muscles
Glycogen Storage Disease Type V
Iodine
Radioactivity
Polyacrylamide Gel Electrophoresis
Coloring Agents
Antibodies
Enzymes

Keywords

  • brain, phosphorylase isoenzymes of human
  • human brain, phosphorylase isoenzymes of
  • isoenzymes, human brain phosphorylase
  • Phosphorylase isoenzymes, from human brain

ASJC Scopus subject areas

  • Clinical Neurology
  • Molecular Biology

Cite this

Bresolin, N., Miranda, A. F., Jacobson, M. P., Lee, J. H., Capilupi, T., & Dimauro, S. (1983). Phosphorylase isoenzymes of human brain. Neurochemical Pathology, 1(3), 171-178. https://doi.org/10.1007/BF02834242

Phosphorylase isoenzymes of human brain. / Bresolin, Nereo; Miranda, Armand F.; Jacobson, Mercedes P.; Lee, Jeen Hyung; Capilupi, Thomas; Dimauro, Salvatore.

In: Neurochemical Pathology, Vol. 1, No. 3, 09.1983, p. 171-178.

Research output: Contribution to journalArticle

Bresolin, N, Miranda, AF, Jacobson, MP, Lee, JH, Capilupi, T & Dimauro, S 1983, 'Phosphorylase isoenzymes of human brain', Neurochemical Pathology, vol. 1, no. 3, pp. 171-178. https://doi.org/10.1007/BF02834242
Bresolin N, Miranda AF, Jacobson MP, Lee JH, Capilupi T, Dimauro S. Phosphorylase isoenzymes of human brain. Neurochemical Pathology. 1983 Sep;1(3):171-178. https://doi.org/10.1007/BF02834242
Bresolin, Nereo ; Miranda, Armand F. ; Jacobson, Mercedes P. ; Lee, Jeen Hyung ; Capilupi, Thomas ; Dimauro, Salvatore. / Phosphorylase isoenzymes of human brain. In: Neurochemical Pathology. 1983 ; Vol. 1, No. 3. pp. 171-178.
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