Phosphorylase isoenzymes of human brain

Nereo Bresolin, Armand F. Miranda, Mercedes P. Jacobson, Jeen Hyung Lee, Thomas Capilupi, Salvatore Dimauro

Research output: Contribution to journalArticlepeer-review

Abstract

The isoenzyme pattern of phosphorylase in human brain was studied by polyacrylamide slab gel electrophoresis and activity was revealed by iodine stain and by autoradiography: A major fast-migrating band was similar to isoenzyme I of the heart; an intermediate band migrated like the hybrid cardiac isoenzyme II; and a slow-migrating band, best revealed by autoradiography, migrated like the "muscle" isoenzyme III. The radioactivity in bands II and III accounted for 42% of total activity in the heart and 37% in the brain. Antibodies against mature muscle phosphorylase inhibited 41% of enzyme activity in heart extracts and 25% in brain extracts. These studies show that the "muscle" isoenzyme is a component of brain phosphorylase and suggest that partial phosphorylase deficiency occurs in the brain of patients with McArdle disease.

Original languageEnglish
Pages (from-to)171-178
Number of pages8
JournalNeurochemical Pathology
Volume1
Issue number3
DOIs
Publication statusPublished - Sep 1983

Keywords

  • brain, phosphorylase isoenzymes of human
  • human brain, phosphorylase isoenzymes of
  • isoenzymes, human brain phosphorylase
  • Phosphorylase isoenzymes, from human brain

ASJC Scopus subject areas

  • Clinical Neurology
  • Molecular Biology

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