Abstract
HIV-1 Rev transactivator is readily phosphorylated at separate regions by protein kinase CK2 and MAP kinase. Protein kinase CK1 cannot replace CK2 as phosphorylating agent and cdc2 only slowly phosphorylates Rev at one of the two sites affected by MAP kinase. Mutational analysis shows that Ser-8 and, to a lesser extent, Ser-5 are phosphorylated by CK2. In contrast, a mutation (R14TV → EED) which suppresses Rev activity dramatically enhances Rev phosphorylation either in vitro by CK2 or in vivo, suggesting that phosphorylation by CK2 could play a role in Rev down-regulation.
| Original language | English |
|---|---|
| Pages (from-to) | 547-554 |
| Number of pages | 8 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 226 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Sep 13 1996 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Phosphorylation of HIV-1 Rev protein : Implication of protein kinase CK2 and pro-directed kinases. / Meggio, Flavio; D'Agostino, Donna M.; Ciminale, Vincenzo; Chieco-Bianchi, Luigi; Pinna, Lorenzo A.
In: Biochemical and Biophysical Research Communications, Vol. 226, No. 2, 13.09.1996, p. 547-554.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Phosphorylation of HIV-1 Rev protein
T2 - Implication of protein kinase CK2 and pro-directed kinases
AU - Meggio, Flavio
AU - D'Agostino, Donna M.
AU - Ciminale, Vincenzo
AU - Chieco-Bianchi, Luigi
AU - Pinna, Lorenzo A.
PY - 1996/9/13
Y1 - 1996/9/13
N2 - HIV-1 Rev transactivator is readily phosphorylated at separate regions by protein kinase CK2 and MAP kinase. Protein kinase CK1 cannot replace CK2 as phosphorylating agent and cdc2 only slowly phosphorylates Rev at one of the two sites affected by MAP kinase. Mutational analysis shows that Ser-8 and, to a lesser extent, Ser-5 are phosphorylated by CK2. In contrast, a mutation (R14TV → EED) which suppresses Rev activity dramatically enhances Rev phosphorylation either in vitro by CK2 or in vivo, suggesting that phosphorylation by CK2 could play a role in Rev down-regulation.
AB - HIV-1 Rev transactivator is readily phosphorylated at separate regions by protein kinase CK2 and MAP kinase. Protein kinase CK1 cannot replace CK2 as phosphorylating agent and cdc2 only slowly phosphorylates Rev at one of the two sites affected by MAP kinase. Mutational analysis shows that Ser-8 and, to a lesser extent, Ser-5 are phosphorylated by CK2. In contrast, a mutation (R14TV → EED) which suppresses Rev activity dramatically enhances Rev phosphorylation either in vitro by CK2 or in vivo, suggesting that phosphorylation by CK2 could play a role in Rev down-regulation.
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UR - http://www.scopus.com/inward/citedby.url?scp=0030582365&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1996.1392
DO - 10.1006/bbrc.1996.1392
M3 - Article
C2 - 8806671
AN - SCOPUS:0030582365
VL - 226
SP - 547
EP - 554
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -