Phosphorylation of HIV-1 Rev protein: Implication of protein kinase CK2 and pro-directed kinases

Flavio Meggio, Donna M. D'Agostino, Vincenzo Ciminale, Luigi Chieco-Bianchi, Lorenzo A. Pinna

Research output: Contribution to journalArticle

Abstract

HIV-1 Rev transactivator is readily phosphorylated at separate regions by protein kinase CK2 and MAP kinase. Protein kinase CK1 cannot replace CK2 as phosphorylating agent and cdc2 only slowly phosphorylates Rev at one of the two sites affected by MAP kinase. Mutational analysis shows that Ser-8 and, to a lesser extent, Ser-5 are phosphorylated by CK2. In contrast, a mutation (R14TV → EED) which suppresses Rev activity dramatically enhances Rev phosphorylation either in vitro by CK2 or in vivo, suggesting that phosphorylation by CK2 could play a role in Rev down-regulation.

Original languageEnglish
Pages (from-to)547-554
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume226
Issue number2
DOIs
Publication statusPublished - Sep 13 1996

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ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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