TY - JOUR
T1 - Phosphorylation of S845 GluA1 AMPA receptors modulates spatial memory and structural plasticity in the ventral striatum
AU - Ferretti, Valentina
AU - Perri, Valentina
AU - Cristofoli, Alessia
AU - Vetere, Gisella
AU - Fragapane, Paola
AU - Oliverio, Alberto
AU - Teule, Martine Ammassari
AU - Mele, Andrea
PY - 2014/6/19
Y1 - 2014/6/19
N2 - The function of AMPA receptors phosphorylation in synaptic plasticity has been dissected in many in vitro models but its role and dynamics on experience-dependent plasticity are still unclear. Here we studied the effects of AMPA receptor manipulations in the ventral striatum, where glutamatergic transmission is known to mediate spatial memory. We first demonstrate that intra-ventral striatal administrations of the AMPA receptors blocker, NBQX, dose dependently impair performance in the Morris water maze. We also report that spatial learning induced a time-limited increase in GluA1 phosphorylation in this same brain region. Finally, through focal, time-controlled ventral striatal administrations of an RNA aptamer interfering with GluA1-S845 phosphorylation, we demonstrate that phosphorylation at this site is a necessary requirement for spatial memory formation and for the synaptic remodeling underlying it. These results suggest that modulation of AMPA receptors by S845 phosphorylation could act as an essential starting signal leading to long-term stabilization of spatial memories.
AB - The function of AMPA receptors phosphorylation in synaptic plasticity has been dissected in many in vitro models but its role and dynamics on experience-dependent plasticity are still unclear. Here we studied the effects of AMPA receptor manipulations in the ventral striatum, where glutamatergic transmission is known to mediate spatial memory. We first demonstrate that intra-ventral striatal administrations of the AMPA receptors blocker, NBQX, dose dependently impair performance in the Morris water maze. We also report that spatial learning induced a time-limited increase in GluA1 phosphorylation in this same brain region. Finally, through focal, time-controlled ventral striatal administrations of an RNA aptamer interfering with GluA1-S845 phosphorylation, we demonstrate that phosphorylation at this site is a necessary requirement for spatial memory formation and for the synaptic remodeling underlying it. These results suggest that modulation of AMPA receptors by S845 phosphorylation could act as an essential starting signal leading to long-term stabilization of spatial memories.
KW - Dendritic spines
KW - NBQX
KW - Nucleus accumbens
KW - RNA aptamer
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U2 - 10.1007/s00429-014-0816-7
DO - 10.1007/s00429-014-0816-7
M3 - Article
C2 - 24942137
AN - SCOPUS:84940591516
VL - 220
SP - 2653
EP - 2661
JO - Zeitschrift fur Anatomie und Entwicklungsgeschichte
JF - Zeitschrift fur Anatomie und Entwicklungsgeschichte
SN - 0177-5154
IS - 5
ER -