Phosphorylation of TCTP as a marker for polo-like kinase-1 activity in vivo

Ulisse Cucchi, Laura M. Gianellini, Anna De Ponti, Francesco Sola, Rachele Alzani, Veronica Patton, Alice Pezzoni, Sonia Troiani, Maria B. Saccardo, Simona Rizzi, Maria L. Giorgini, Paolo Cappella, Italo Beria, Barbara Valsasina

Research output: Contribution to journalArticle

Abstract

Polo-like kinase 1 (PLK1) is the master regulator of mitosis and a target for anticancer therapy. To develop a marker of PLK1 activity in cells and tumour tissues, this study focused on translational controlled tumour protein (TCTP) and identified serine 46 as a site phosphorylated by PLK1 in vitro. Using an antibody raised against phosphoTCTP-Ser46, it was demonstrated that phosphorylation at this site correlates with PLK1 level and kinase activity in cells. Moreover, PLK1 depletion by siRNA or inactivation by specific inhibitors caused a correspondent decrease in phospho-TCTP-Ser46 signal validating this site as a direct marker of PLK1. Using this marker, the study characterized PLK1 inhibitors in cells by setting up a high-content assay and finally immunohistochemical assay suitable for following inhibitor activity in preclinical tumour models and possibly in clinical studies was developed.

Original languageEnglish
Pages (from-to)4973-4986
Number of pages14
JournalAnticancer Research
Volume30
Issue number12
Publication statusPublished - Dec 2010

Keywords

  • Antitumor activity
  • Biomarker
  • PLK1
  • TCTP

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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    Cucchi, U., Gianellini, L. M., De Ponti, A., Sola, F., Alzani, R., Patton, V., Pezzoni, A., Troiani, S., Saccardo, M. B., Rizzi, S., Giorgini, M. L., Cappella, P., Beria, I., & Valsasina, B. (2010). Phosphorylation of TCTP as a marker for polo-like kinase-1 activity in vivo. Anticancer Research, 30(12), 4973-4986.