Phosphorylation of VE-cadherin is modulated by haemodynamic forces and contributes to the regulation of vascular permeability in vivo

Fabrizio Orsenigo, Costanza Giampietro, Aldo Ferrari, Monica Corada, Ariane Galaup, Sara Sigismund, Giuseppe Ristagno, Luigi Maddaluno, Gou Young Koh, Davide Franco, Vartan Kurtcuoglu, Dimos Poulikakos, Peter Baluk, Donald McDonald, Maria Grazia Lampugnani, Elisabetta Dejana

Research output: Contribution to journalArticle

205 Citations (Scopus)

Abstract

Endothelial adherens junctions maintain vascular integrity. Arteries and veins differ in their permeability but whether organization and strength of their adherens junctions vary has not been demonstrated in vivo. Here we report that vascular endothelial cadherin, an endothelial specific adhesion protein located at adherens junctions, is phosphorylated in Y658 and Y685 in vivo in veins but not in arteries under resting conditions. This difference is due to shear stress-induced junctional Src activation in veins. Phosphorylated vascular endothelial-cadherin is internalized and ubiquitinated in response to permeability-increasing agents such as bradykinin and histamine. Inhibition of Src blocks vascular endothelial cadherin phosphorylation and bradykinin-induced permeability. Point mutation of Y658F and Y685F prevents vascular endothelial cadherin internalization, ubiquitination and an increase in permeability by bradykinin in vitro. Thus, phosphorylation of vascular endothelial cadherin contributes to a dynamic state of adherens junctions, but is not sufficient to increase vascular permeability in the absence of inflammatory agents.

Original languageEnglish
Article number1208
JournalNature Communications
Volume3
DOIs
Publication statusPublished - 2012

Fingerprint

phosphorylation
Phosphorylation
hemodynamics
Hemodynamics
Capillary Permeability
Adherens Junctions
permeability
Permeability
Bradykinin
veins
Veins
arteries
Arteries
histamines
Ubiquitination
mutations
Point Mutation
integrity
Histamine
shear stress

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Phosphorylation of VE-cadherin is modulated by haemodynamic forces and contributes to the regulation of vascular permeability in vivo. / Orsenigo, Fabrizio; Giampietro, Costanza; Ferrari, Aldo; Corada, Monica; Galaup, Ariane; Sigismund, Sara; Ristagno, Giuseppe; Maddaluno, Luigi; Koh, Gou Young; Franco, Davide; Kurtcuoglu, Vartan; Poulikakos, Dimos; Baluk, Peter; McDonald, Donald; Grazia Lampugnani, Maria; Dejana, Elisabetta.

In: Nature Communications, Vol. 3, 1208, 2012.

Research output: Contribution to journalArticle

Orsenigo, F, Giampietro, C, Ferrari, A, Corada, M, Galaup, A, Sigismund, S, Ristagno, G, Maddaluno, L, Koh, GY, Franco, D, Kurtcuoglu, V, Poulikakos, D, Baluk, P, McDonald, D, Grazia Lampugnani, M & Dejana, E 2012, 'Phosphorylation of VE-cadherin is modulated by haemodynamic forces and contributes to the regulation of vascular permeability in vivo', Nature Communications, vol. 3, 1208. https://doi.org/10.1038/ncomms2199
Orsenigo, Fabrizio ; Giampietro, Costanza ; Ferrari, Aldo ; Corada, Monica ; Galaup, Ariane ; Sigismund, Sara ; Ristagno, Giuseppe ; Maddaluno, Luigi ; Koh, Gou Young ; Franco, Davide ; Kurtcuoglu, Vartan ; Poulikakos, Dimos ; Baluk, Peter ; McDonald, Donald ; Grazia Lampugnani, Maria ; Dejana, Elisabetta. / Phosphorylation of VE-cadherin is modulated by haemodynamic forces and contributes to the regulation of vascular permeability in vivo. In: Nature Communications. 2012 ; Vol. 3.
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