Photo-induced electron transfer real-time PCR for detection of plasmodium falciparum plasmepsin 2 gene copy number

Samaly Santos Souza; Mariangela L’Episcopia; Carlo Severini; Venkatachalam Udhayakumar; Naomi W. Lucchi

doi:10.1128/AAC.00317-18

Photo-induced electron transfer real-time PCR for detection of plasmodium falciparum plasmepsin 2 gene copy number

Samaly Santos Souza, Mariangela L’Episcopia, Carlo Severini, Venkatachalam Udhayakumar, Naomi W. Lucchi

Istituto Superiore di Sanita'

Research output: Contribution to journal › Article › peer-review

Abstract

Piperaquine is an important partner drug used in artemisinin-based combination therapies (ACTs). An increase in the plasmepsin 2 and 3 gene copy numbers has been associated with decreased susceptibility of Plasmodium falciparum to piperaquine in Cambodia. Here, we developed a photo-induced electron transfer real-time PCR (PET-PCR) assay to quantify the copy number of the P. falciparum plasmepsin 2 gene (PfPM2) that can be used in countries where P. falciparum is endemic to enhance molecular surveillance.

Original language	English
Article number	e00317-18
Journal	Antimicrobial Agents and Chemotherapy
Volume	62
Issue number	8
DOIs	https://doi.org/10.1128/AAC.00317-18
Publication status	Published - Aug 1 2018

Keywords

Copy number
Malaria
PET-PCR
Piperaquine

ASJC Scopus subject areas

Pharmacology
Pharmacology (medical)
Infectious Diseases

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10.1128/AAC.00317-18

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Photo-induced electron transfer real-time PCR for detection of plasmodium falciparum plasmepsin 2 gene copy number. / Souza, Samaly Santos; L’Episcopia, Mariangela; Severini, Carlo; Udhayakumar, Venkatachalam; Lucchi, Naomi W.

In: Antimicrobial Agents and Chemotherapy, Vol. 62, No. 8, e00317-18, 01.08.2018.

Research output: Contribution to journal › Article › peer-review

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