Photo-induced electron transfer real-time PCR for detection of plasmodium falciparum plasmepsin 2 gene copy number

Samaly Santos Souza, Mariangela L’Episcopia, Carlo Severini, Venkatachalam Udhayakumar, Naomi W. Lucchi

Research output: Contribution to journalArticlepeer-review

Abstract

Piperaquine is an important partner drug used in artemisinin-based combination therapies (ACTs). An increase in the plasmepsin 2 and 3 gene copy numbers has been associated with decreased susceptibility of Plasmodium falciparum to piperaquine in Cambodia. Here, we developed a photo-induced electron transfer real-time PCR (PET-PCR) assay to quantify the copy number of the P. falciparum plasmepsin 2 gene (PfPM2) that can be used in countries where P. falciparum is endemic to enhance molecular surveillance.

Original languageEnglish
Article numbere00317-18
JournalAntimicrobial Agents and Chemotherapy
Volume62
Issue number8
DOIs
Publication statusPublished - Aug 1 2018

Keywords

  • Copy number
  • Malaria
  • PET-PCR
  • Piperaquine

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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