TY - JOUR
T1 - Photo-induced electron transfer real-time PCR for detection of plasmodium falciparum plasmepsin 2 gene copy number
AU - Souza, Samaly Santos
AU - L’Episcopia, Mariangela
AU - Severini, Carlo
AU - Udhayakumar, Venkatachalam
AU - Lucchi, Naomi W.
PY - 2018/8/1
Y1 - 2018/8/1
N2 - Piperaquine is an important partner drug used in artemisinin-based combination therapies (ACTs). An increase in the plasmepsin 2 and 3 gene copy numbers has been associated with decreased susceptibility of Plasmodium falciparum to piperaquine in Cambodia. Here, we developed a photo-induced electron transfer real-time PCR (PET-PCR) assay to quantify the copy number of the P. falciparum plasmepsin 2 gene (PfPM2) that can be used in countries where P. falciparum is endemic to enhance molecular surveillance.
AB - Piperaquine is an important partner drug used in artemisinin-based combination therapies (ACTs). An increase in the plasmepsin 2 and 3 gene copy numbers has been associated with decreased susceptibility of Plasmodium falciparum to piperaquine in Cambodia. Here, we developed a photo-induced electron transfer real-time PCR (PET-PCR) assay to quantify the copy number of the P. falciparum plasmepsin 2 gene (PfPM2) that can be used in countries where P. falciparum is endemic to enhance molecular surveillance.
KW - Copy number
KW - Malaria
KW - PET-PCR
KW - Piperaquine
UR - http://www.scopus.com/inward/record.url?scp=85052016014&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85052016014&partnerID=8YFLogxK
U2 - 10.1128/AAC.00317-18
DO - 10.1128/AAC.00317-18
M3 - Article
C2 - 29866871
AN - SCOPUS:85052016014
VL - 62
JO - Antimicrobial Agents and Chemotherapy
JF - Antimicrobial Agents and Chemotherapy
SN - 0066-4804
IS - 8
M1 - e00317-18
ER -