Photobiomodulation with low-level diode laser promotes osteoblast migration in an in vitro micro wound model

Matilde Tschon, Serena Incerti-Parenti, Simona Cepollaro, Luigi Checchi, Milena Fini

Research output: Contribution to journalArticle

Abstract

Laser photobiomodulation can improve bone healing, but well-defined treatment parameters are lacking. Saos-2 human osteoblast-like cells were subjected to an in vitro scratch-wound healing assay and irradiated by a 915-nm gallium-aluminum-arsenide diode laser for 0, 48, 96, and 144 s using doses of, respectively, 0, 5, 10, and 15 J/cm2. Wound area was measured after 4, 24, 48, and 72 h. Cell viability, DNA content, gene expression, and release of bone-related proteins were evaluated after 24, 48, and 72 h. Laser significantly improved wound healing compared with nonirradiated controls. Cells treated with laser doses of 5 and 10 J/cm2 reached wound closure after 72 h, followed by 15 J/cm2 after 96 h. With the cell proliferation inhibitor Mitomycin C, the doses of 10 and 15 J/cm2 maintained an improved wound healing compared with controls. Laser increased collagen type 1 gene expression with higher doses inducing a longer-lasting effect, whereas transforming growth factor-beta 1 showed comparable or decreased levels in irradiated versus nonirradiated groups, with no effect on protein release. This study demonstrated that laser photobiomodulation at 915 nm promoted wound healing mainly through stimulation of cell migration and collagen deposition by osteoblasts.

Original languageEnglish
Article number078002
JournalJournal of Biomedical Optics
Volume20
Issue number7
DOIs
Publication statusPublished - Jul 2 2015

Fingerprint

osteoblasts
Osteoblasts
wound healing
Semiconductor lasers
semiconductor lasers
Lasers
dosage
gene expression
lasers
collagens
Collagen
Gene expression
bones
Aluminum gallium arsenide
Bone
cells
proteins
Proteins
beta factor
healing

Keywords

  • diode laser
  • in vitro scratch assay
  • low-level laser therapy
  • osteoblast-like cells
  • photobiomodulation
  • wound healing

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Photobiomodulation with low-level diode laser promotes osteoblast migration in an in vitro micro wound model. / Tschon, Matilde; Incerti-Parenti, Serena; Cepollaro, Simona; Checchi, Luigi; Fini, Milena.

In: Journal of Biomedical Optics, Vol. 20, No. 7, 078002, 02.07.2015.

Research output: Contribution to journalArticle

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