Abstract
Laser photobiomodulation can improve bone healing, but well-defined treatment parameters are lacking. Saos-2 human osteoblast-like cells were subjected to an in vitro scratch-wound healing assay and irradiated by a 915-nm gallium-aluminum-arsenide diode laser for 0, 48, 96, and 144 s using doses of, respectively, 0, 5, 10, and 15 J/cm2. Wound area was measured after 4, 24, 48, and 72 h. Cell viability, DNA content, gene expression, and release of bone-related proteins were evaluated after 24, 48, and 72 h. Laser significantly improved wound healing compared with nonirradiated controls. Cells treated with laser doses of 5 and 10 J/cm2 reached wound closure after 72 h, followed by 15 J/cm2 after 96 h. With the cell proliferation inhibitor Mitomycin C, the doses of 10 and 15 J/cm2 maintained an improved wound healing compared with controls. Laser increased collagen type 1 gene expression with higher doses inducing a longer-lasting effect, whereas transforming growth factor-beta 1 showed comparable or decreased levels in irradiated versus nonirradiated groups, with no effect on protein release. This study demonstrated that laser photobiomodulation at 915 nm promoted wound healing mainly through stimulation of cell migration and collagen deposition by osteoblasts.
| Original language | English |
|---|---|
| Article number | 078002 |
| Journal | Journal of Biomedical Optics |
| Volume | 20 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Jul 2 2015 |
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Keywords
- diode laser
- in vitro scratch assay
- low-level laser therapy
- osteoblast-like cells
- photobiomodulation
- wound healing
ASJC Scopus subject areas
- Biomedical Engineering
- Biomaterials
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
Cite this
Photobiomodulation with low-level diode laser promotes osteoblast migration in an in vitro micro wound model. / Tschon, Matilde; Incerti-Parenti, Serena; Cepollaro, Simona; Checchi, Luigi; Fini, Milena.
In: Journal of Biomedical Optics, Vol. 20, No. 7, 078002, 02.07.2015.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Photobiomodulation with low-level diode laser promotes osteoblast migration in an in vitro micro wound model
AU - Tschon, Matilde
AU - Incerti-Parenti, Serena
AU - Cepollaro, Simona
AU - Checchi, Luigi
AU - Fini, Milena
PY - 2015/7/2
Y1 - 2015/7/2
N2 - Laser photobiomodulation can improve bone healing, but well-defined treatment parameters are lacking. Saos-2 human osteoblast-like cells were subjected to an in vitro scratch-wound healing assay and irradiated by a 915-nm gallium-aluminum-arsenide diode laser for 0, 48, 96, and 144 s using doses of, respectively, 0, 5, 10, and 15 J/cm2. Wound area was measured after 4, 24, 48, and 72 h. Cell viability, DNA content, gene expression, and release of bone-related proteins were evaluated after 24, 48, and 72 h. Laser significantly improved wound healing compared with nonirradiated controls. Cells treated with laser doses of 5 and 10 J/cm2 reached wound closure after 72 h, followed by 15 J/cm2 after 96 h. With the cell proliferation inhibitor Mitomycin C, the doses of 10 and 15 J/cm2 maintained an improved wound healing compared with controls. Laser increased collagen type 1 gene expression with higher doses inducing a longer-lasting effect, whereas transforming growth factor-beta 1 showed comparable or decreased levels in irradiated versus nonirradiated groups, with no effect on protein release. This study demonstrated that laser photobiomodulation at 915 nm promoted wound healing mainly through stimulation of cell migration and collagen deposition by osteoblasts.
AB - Laser photobiomodulation can improve bone healing, but well-defined treatment parameters are lacking. Saos-2 human osteoblast-like cells were subjected to an in vitro scratch-wound healing assay and irradiated by a 915-nm gallium-aluminum-arsenide diode laser for 0, 48, 96, and 144 s using doses of, respectively, 0, 5, 10, and 15 J/cm2. Wound area was measured after 4, 24, 48, and 72 h. Cell viability, DNA content, gene expression, and release of bone-related proteins were evaluated after 24, 48, and 72 h. Laser significantly improved wound healing compared with nonirradiated controls. Cells treated with laser doses of 5 and 10 J/cm2 reached wound closure after 72 h, followed by 15 J/cm2 after 96 h. With the cell proliferation inhibitor Mitomycin C, the doses of 10 and 15 J/cm2 maintained an improved wound healing compared with controls. Laser increased collagen type 1 gene expression with higher doses inducing a longer-lasting effect, whereas transforming growth factor-beta 1 showed comparable or decreased levels in irradiated versus nonirradiated groups, with no effect on protein release. This study demonstrated that laser photobiomodulation at 915 nm promoted wound healing mainly through stimulation of cell migration and collagen deposition by osteoblasts.
KW - diode laser
KW - in vitro scratch assay
KW - low-level laser therapy
KW - osteoblast-like cells
KW - photobiomodulation
KW - wound healing
UR - http://www.scopus.com/inward/record.url?scp=84947983124&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84947983124&partnerID=8YFLogxK
U2 - 10.1117/1.JBO.20.7.078002
DO - 10.1117/1.JBO.20.7.078002
M3 - Article
C2 - 26140461
AN - SCOPUS:84947983124
VL - 20
JO - Journal of Biomedical Optics
JF - Journal of Biomedical Optics
SN - 1083-3668
IS - 7
M1 - 078002
ER -