Photolithography and micromolding techniques for the realization of 3D polycaprolactone scaffolds for tissue engineering applications

Tania Limongi, Rossana Schipani, Anna Di Vito, Andrea Giugni, Marco Francardi, Bruno Torre, Marco Allione, Ermanno Miele, Natalia Malara, Salma Alrasheed, Raffaella Raimondo, Patrizio Candeloro, Vincenzo Mollace, Enzo Di Fabrizio

Research output: Contribution to journalArticle

Abstract

Material science, cell biology, and engineering are all part of the research field of tissue engineering. It is the application of knowledge, methods and instrumentations of engineering and life science to the development of biocompatible solutions for repair and/or replace tissues and damaged organs. Last generation microfabrication technologies utilizing natural and synthetic biomaterials allow the realization of scaffolds resembling tissue-like structures as skin, brain, bones, muscles, cartilage and blood vessels. In this work we describe an effective and simple micromolding fabrication process allowing the realization of 3D polycaprolactone (PCL) scaffold for human neural stem cells (hNSC) culture. Scanning Electron Microscopy has been used to investigate the micro and nano features characterizing the surface of the device. Immunofluorescence analysis showed how, after seeding cells onto the substrate, healthy astrocytes grew up in a well-organized 3D network. Thus, we proposed this effective fabrication method for the production of nanopatterned PCL pillared scaffold providing a biomimetic environment for the growth of hNSC, a promising and efficient means for future applications in tissue engineering and regenerative medicine.

Original languageEnglish
Pages (from-to)135-139
Number of pages5
JournalMicroelectronic Engineering
Volume141
DOIs
Publication statusPublished - Jun 15 2015

Keywords

  • Biocompatible substrate
  • Human stem cells
  • Microfabrication technique
  • Nanostructured PCL pillars
  • Neural networks

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Surfaces, Coatings and Films
  • Atomic and Molecular Physics, and Optics
  • Condensed Matter Physics

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  • Cite this

    Limongi, T., Schipani, R., Di Vito, A., Giugni, A., Francardi, M., Torre, B., Allione, M., Miele, E., Malara, N., Alrasheed, S., Raimondo, R., Candeloro, P., Mollace, V., & Di Fabrizio, E. (2015). Photolithography and micromolding techniques for the realization of 3D polycaprolactone scaffolds for tissue engineering applications. Microelectronic Engineering, 141, 135-139. https://doi.org/10.1016/j.mee.2015.02.030