Phycocyanobilin promotes PC12 cell survival and modulates immune and inflammatory genes and oxidative stress markers in acute cerebral hypoperfusion in rats

Javier Marín-Prida, Nancy Pavón-Fuentes, Alexey Llópiz-Arzuaga, Julio R. Fernández-Massó, Liván Delgado-Roche, Yssel Mendoza-Marí, Seydi Pedroso Santana, Alieski Cruz-Ramírez, Carmen Valenzuela-Silva, Marcelo Nazábal-Gálvez, Alberto Cintado-Benítez, Gilberto L. Pardo-Andreu, Nadia Polentarutti, Federica Riva, Eduardo Pentón-Arias, Giselle Pentón-Rol

Research output: Contribution to journalArticle

Abstract

Since the inflammatory response and oxidative stress are involved in the stroke cascade, we evaluated here the effects of Phycocyanobilin (PCB, the C-Phycocyanin linked tetrapyrrole) on PC12 cell survival, the gene expression and the oxidative status of hypoperfused rat brain. After the permanent bilateral common carotid arteries occlusion (BCCAo), the animals were treated with saline or PCB, taking samples 24h post-surgery. Global gene expression was analyzed with GeneChip Rat Gene ST 1.1 from Affymetrix; the expression of particular genes was assessed by the Fast SYBR Green RT-PCR Master Mix and Bioplex methods; and redox markers (MDA, PP, CAT, SOD) were evaluated spectrophotometrically. The PCB treatment prevented the H2O2 and glutamate induced PC12 cell injury assessed by the MTT assay, and modulated 190 genes (93 up- and 97 down-regulated) associated to several immunological and inflammatory processes in BCCAo rats. Furthermore, PCB positively modulated 19 genes mostly related to a detrimental pro-inflammatory environment and counteracted the oxidative imbalance in the treated BCCAo animals. Our results support the view of an effective influence of PCB on major inflammatory mediators in acute cerebral hypoperfusion. These results suggest that PCB has a potential to be a treatment for ischemic stroke for which further studies are needed.

Original languageEnglish
Pages (from-to)49-60
Number of pages12
JournalToxicology and Applied Pharmacology
Volume272
Issue number1
DOIs
Publication statusPublished - Oct 1 2013

Keywords

  • Cerebral hypoperfusion
  • Gene expression
  • Inflammation
  • Oxidative stress
  • PC12
  • Phycocyanobilin

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

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    Marín-Prida, J., Pavón-Fuentes, N., Llópiz-Arzuaga, A., Fernández-Massó, J. R., Delgado-Roche, L., Mendoza-Marí, Y., Santana, S. P., Cruz-Ramírez, A., Valenzuela-Silva, C., Nazábal-Gálvez, M., Cintado-Benítez, A., Pardo-Andreu, G. L., Polentarutti, N., Riva, F., Pentón-Arias, E., & Pentón-Rol, G. (2013). Phycocyanobilin promotes PC12 cell survival and modulates immune and inflammatory genes and oxidative stress markers in acute cerebral hypoperfusion in rats. Toxicology and Applied Pharmacology, 272(1), 49-60. https://doi.org/10.1016/j.taap.2013.05.021