Phylogenetic analysis of two genotype 3 Hepatitis E viruses from wild boar, Italy

Luca De Sabato, Gabriele Vaccari, Philippe Lemey, Maria Grazia Amoroso, Giovanna Fusco, Giovanni Ianiro, Ilaria Di Bartolo

Research output: Contribution to journalArticlepeer-review


The complete and near-complete genome sequences (7206 nt and 7229 nt) of two wild boar HEV strains detected in Southern Italy were obtained by the next generation sequencing. Phylogenetic analysis and p distance comparisons of one of the strains with HEV-3 reference subtype strains confirmed the detection of a subtype 3i (p distance = 0.110) strain in wild boar, never detected in Italy either in wild boar or pigs. The sequence of the second strain was not classifiable in any of the subtypes defined to date, showing a p distance > 0.138 and a low nucleotide identity with all HEV reference strains. The virus may represent a novel subtype, with a low relationship to other strains of genotype 3 detected in wild boar, pigs, or humans in Europe. This result suggests the circulation in Italy of an emerging or uncommon HEV strain. Sequencing followed by phylogenetic analyses of the complete HEV coding regions are important tools for understanding the evolutionary and epidemiological dynamics underlying the wide genetic diversity of HEV strains.

Original languageEnglish
Pages (from-to)812-817
Number of pages6
JournalVirus Genes
Issue number6
Publication statusPublished - Dec 1 2018


  • Full genome
  • Hepatitis E virus
  • HEV-3
  • Next generation sequencing
  • Subtype
  • Wild boar

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Virology


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