TY - JOUR
T1 - Phylogenetic and metabolic tracking of gut microbiota during perinatal development
AU - Del Chierico, Federica
AU - Vernocchi, Pamela
AU - Petrucca, Andrea
AU - Paci, Paola
AU - Fuentes, Susana
AU - Praticò, Giulia
AU - Capuani, Giorgio
AU - Masotti, Andrea
AU - Reddel, Sofia
AU - Russo, Alessandra
AU - Vallone, Cristina
AU - Salvatori, Guglielmo
AU - Buffone, Elsa
AU - Signore, Fabrizio
AU - Rigon, Giuliano
AU - Dotta, Andrea
AU - Miccheli, Alfredo
AU - De Vos, Willem M.
AU - Dallapiccola, Bruno
AU - Putignani, Lorenza
PY - 2015/9/2
Y1 - 2015/9/2
N2 - The colonization and development of gut microbiota immediately after birth is highly variable and depends on several factors, such as delivery mode and modality of feeding during the first months of life. A cohort of 31 mother and neonate pairs, including 25 at-term caesarean (CS) and 6 vaginally (V) delivered neonates (DNs), were included in this study and 121 meconium/faecal samples were collected at days 1 through 30 following birth. Operational taxonomic units (OTUs) were assessed in 69 stool samples by phylogenetic microarray HITChip and inter- and intra-individual distributions were established by inter-OTUs correlation matrices and OTUs co-occurrence or co-exclusion networks. 1H-NMR metabolites were determined in 70 stool samples, PCA analysis was performed on 55 CS DNs samples, and metabolome/OTUs co-correlations were assessed in 45 CS samples, providing an integrated map of the early microbiota OTUs-metabolome. A microbiota "core" of OTUs was identified that was independent of delivery mode and lactation stage, suggesting highly specialized communities that act as seminal colonizers of microbial networks. Correlations among OTUs, metabolites, and OTUs-metabolites revealed metabolic profiles associated with early microbial ecological dynamics, maturation of milk components, and host physiology.
AB - The colonization and development of gut microbiota immediately after birth is highly variable and depends on several factors, such as delivery mode and modality of feeding during the first months of life. A cohort of 31 mother and neonate pairs, including 25 at-term caesarean (CS) and 6 vaginally (V) delivered neonates (DNs), were included in this study and 121 meconium/faecal samples were collected at days 1 through 30 following birth. Operational taxonomic units (OTUs) were assessed in 69 stool samples by phylogenetic microarray HITChip and inter- and intra-individual distributions were established by inter-OTUs correlation matrices and OTUs co-occurrence or co-exclusion networks. 1H-NMR metabolites were determined in 70 stool samples, PCA analysis was performed on 55 CS DNs samples, and metabolome/OTUs co-correlations were assessed in 45 CS samples, providing an integrated map of the early microbiota OTUs-metabolome. A microbiota "core" of OTUs was identified that was independent of delivery mode and lactation stage, suggesting highly specialized communities that act as seminal colonizers of microbial networks. Correlations among OTUs, metabolites, and OTUs-metabolites revealed metabolic profiles associated with early microbial ecological dynamics, maturation of milk components, and host physiology.
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U2 - 10.1371/journal.pone.0137347
DO - 10.1371/journal.pone.0137347
M3 - Article
AN - SCOPUS:84947475253
VL - 10
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 9
M1 - e0137347
ER -