Phylogenetic characterization of enterovirus 68 strains in patients with respiratory syndromes in Italy

Antonio Piralla, Alessia Girello, Michela Grignani, Monica Gozalo-Margüello, Antonietta Marchi, Gianluigi Marseglia, Fausto Baldanti

Research output: Contribution to journalArticlepeer-review

Abstract

Enterovirus 68 (EV-D68) was associated with mild to severe respiratory infections. In the last 4 years, circulation of different EV-D68 strains has been documented worldwide. In this study, the phylogenetic characterization of nine EV-D68 strains identified in patients in the 2010-2012 period and 12 additional EV-D68 Italian strains previously identified in 2008 in Italy was described. From January 2010 to December 2012, a total of 889 respiratory specimens from 588 patients stayed or visited at the Fondazione IRCCS Policlinico San Matteo were positive for HRV or HEV. Extracted nucleic acids were amplified by one-step RT-PCR with primer specific for VP1 region of EV-D68 and purified positive PCR products were directly sequenced. Overall, 9/3736 (0.24%) patients were EV-D68 positive. Of these, 7/9 (77.8%) were pediatric and two (22.2%) were adults. Five out of seven (71.4%) pediatric patients had lower respiratory tract infection with oxygen saturation

Original languageEnglish
Pages (from-to)1590-1593
Number of pages4
JournalJournal of Medical Virology
Volume86
Issue number9
DOIs
Publication statusPublished - 2014

Keywords

  • Enterovirus 68
  • Italian strains
  • Respiratory infection
  • VP1 sequences

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Medicine(all)

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