Phylogenetic lineages, clones and β-lactamases in an international collection of Klebsiella oxytoca isolates non-susceptible to expanded-spectrum cephalosporins

R. Izdebski, J. Fiett, P. Urbanowicz, A. Baraniak, L. P G Derde, M. J M Bonten, Y. Carmeli, H. Goossens, W. Hryniewicz, C. Brun-Buisson, S. Brisse, M. Gniadkowski, M. Herda, M. J. Dautzenberg, A. Adler, M. Kazma, S. Navon-Venezia, S. Malhotra-Kumar, C. Lammens, P. LegrandA. Chalfine, H. Giamarellou, G. L. Petrikkos, A. Balode, U. Dumpis, P. Stammet, I. Aragăo, F. Esteves, A. Torres Martí, C. Lawrence, J. Salomon, M. Paul, Y. Lerman, A. Rossini, A. Salvia, J. Vidal Samso, J. Fierro

Research output: Contribution to journalArticlepeer-review

Abstract

Objectives: The objective of this study was to examine Klebsiella oxytoca clonal and phylogenetic diversity, based on an international collection of carriage isolates non-susceptible to expanded-spectrum cephalosporins (ESCs). Methods: The study material comprised 68 rectal carriage K. oxytoca isolates non-susceptible to ESCs recovered in 2008-11 from patients in 14 hospitals across Europe and Israel. ESC resistance was tested phenotypically; genes encoding ESBLs, AmpC cephalosporinases and carbapenemases were amplified and sequenced. The isolates were typed by PFGE and MLST, followed by sequencing of blaOXY genes. Results: MLSTand PFGE distinguished 34 STs and 47 pulsotypes among the isolates, respectively. Six STswere split into several pulsotypes each. Five STs were more prevalent (n=2-9) and occurred in several countries each, including ST2, ST9 and ST141, which belong to a growing international clonal complex (CC), CC2. Four phylogenetic lineages were distinguished, each with another type of chromosomal OXY-type β-lactamase. Three of these, with OXY-1/-5, OXY-2 types and OXY-4, corresponded to previously described phylogroups KoI, KoII and KoIV, respectively. A single isolate from Israel represented a distinct lineage with a newly defined OXY-7 type. The phylogroups showed interesting differences in mechanisms of ESC resistance; KoI strains rarely overexpressed the OXY enzymes but commonly produced ESBLs, whereas KoII strains often were OXY hyperproducers and carried ESBLs much less frequently. AmpCs (DHA-1) and carbapenemases (VIM-1) occurred sporadically. Conclusions: The study confirmed the high genetic diversity of the collection of K. oxytoca ESC-non-susceptible isolates, composed of phylogroups with distinct types of OXY-type β-lactamases, and revealed some STs of broad geographical distribution.

Original languageEnglish
Pages (from-to)3230-3237
Number of pages8
JournalJournal of Antimicrobial Chemotherapy
Volume70
Issue number12
DOIs
Publication statusPublished - 2015

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Phylogenetic lineages, clones and β-lactamases in an international collection of Klebsiella oxytoca isolates non-susceptible to expanded-spectrum cephalosporins'. Together they form a unique fingerprint.

Cite this