Phylogeny and regulation of four lipocalin genes clustered in the chicken genome: Evidence of a functional diversification after gene duplication

Aldo Pagano, Paolo Giannoni, Adriana Zambotti, Diego Sánchez, Maria Dolores Ganfornina, Gabriel Gutiérrez, Nadia Randazzo, Ranieri Cancedda, Beatrice Dozin

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A novel lipocalin gene is here reported that represents the fourth member of a cluster we have identified in the chicken genome. This cluster also includes Chondrogenesis-Associated Lipocalins β and γ (CALβ, CALγ) and Extracellular Fatty Acid Binding Protein (Ex-FABP). The new gene codes for a 22-kDa secreted protein with three cysteine residues and a series of sequence features well conserved in the lipocalin family. All the genes in the cluster are structurally similar presenting comparable exon/intron boundary positions and exon sizes. A phylogenetic analysis indicates the monophyletic grouping of these genes, and their relationship with the lipocalins α-1-microglobulin (A1mg), complement factor 8γ chain (C8GC), prostaglandin D synthase (PGDS), and neutrophil-gelatinase-associated lipocalin (NGAL). The new cluster gene appears to be the ortholog of the mammalian C8GC and was thus named Ggal-C8GC. This orthology also suggests that this lipocalin was present in the ancestor common to reptiles and mammals. In addition to other expressing tissues, Ex-FABP, CALβ and CALγ genes are highly transcribed in chondrocytes at late stages of chondrogenesis during endochondral bone formation and/or upon inflammatory stimulation. Here, we show that they are also transcriptionally induced when chondrocytes are subjected to various biological events as cell quiescence, cell shape transition, and hormonal stimulation. By contrast, Ggal-C8GC transcripts are only barely detectable in chondrocytes, but are more abundant in liver, kidney, brain, heart, skeletal muscle and particularly in skin. Moreover, no expression induction was observed neither during chondrocyte differentiation, nor upon any of the stimulations mentioned above. This indicates that the Ggal-C8GC gene was co-opted for a novel function after the duplication events that gave rise to the cluster. The peculiar coordinated regulation of Ex-FABP, CALβ and CALγ, and the apparent divergent role of Ggal-C8GC suggest that these gene duplications may have been maintained during evolution by a sub-functionalization mechanism where some common function(s) are shared by several members of the cluster and some other specialized function(s) are unique to other members.

Original languageEnglish
Pages (from-to)95-106
Number of pages12
JournalGene
Volume331
Issue number1-2
DOIs
Publication statusPublished - Apr 28 2004

Fingerprint

Lipocalins
Gene Duplication
Phylogeny
Chickens
Chondrocytes
Fatty Acid-Binding Proteins
Genome
Chondrogenesis
Genes
prostaglandin R2 D-isomerase
Multigene Family
Complement C8
Lipocalin 1
Exons
Reptiles
Cell Shape
Osteogenesis
Introns
Cysteine
Mammals

Keywords

  • α-1-microglobulin
  • A1mg
  • C8GC
  • CAL
  • Chondrogenesis- associated lipocalin
  • Complement factor 8γ chain
  • Evolution
  • Ex-FABP
  • Extracellular fatty acid binding protein
  • Gene structure
  • NGAL
  • PGDS
  • Prostaglandin D synthase
  • Transcription regulation

ASJC Scopus subject areas

  • Genetics

Cite this

Phylogeny and regulation of four lipocalin genes clustered in the chicken genome : Evidence of a functional diversification after gene duplication. / Pagano, Aldo; Giannoni, Paolo; Zambotti, Adriana; Sánchez, Diego; Ganfornina, Maria Dolores; Gutiérrez, Gabriel; Randazzo, Nadia; Cancedda, Ranieri; Dozin, Beatrice.

In: Gene, Vol. 331, No. 1-2, 28.04.2004, p. 95-106.

Research output: Contribution to journalArticle

Pagano, Aldo ; Giannoni, Paolo ; Zambotti, Adriana ; Sánchez, Diego ; Ganfornina, Maria Dolores ; Gutiérrez, Gabriel ; Randazzo, Nadia ; Cancedda, Ranieri ; Dozin, Beatrice. / Phylogeny and regulation of four lipocalin genes clustered in the chicken genome : Evidence of a functional diversification after gene duplication. In: Gene. 2004 ; Vol. 331, No. 1-2. pp. 95-106.
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