Physical Activity Modulates the Overexpression of the Inflammatory miR-146a-5p in Obese Patients

Angelo Russo, Desireé Bartolini, Emanuela Mensà, Pierangelo Torquato, Maria Cristina Albertini, Fabiola Olivieri, Roberto Testa, Sara Rossi, Marta Piroddi, Gabriele Cruciani, Pierpaolo De Feo, Francesco Galli

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Specific microRNAs (miRs), including the “angio-miR-126” and the “inflamma-miR-146a-5p,” have been proposed as biomarkers and even therapeutic targets of obesity-associated metabolic diseases. Physical activity, a key measure of prevention for obesity and its complications, is reported to influence the expression of these miRs. In this study, we investigate whether a physical activity program proven to improve metabolic parameters in obese patients can correct the circulating levels of these miRs. Plasma miR-126 and miR-146a-5p were measured in a cohort of obese patients (n = 31, 16F + 15M) before and after the 3-month physical activity program of the CURIAMO trial (registration number for clinical trials: ACTRN12611000255987) and in 37 lean controls (24F + 13M). miR-146a-5p, but not miR-126, was significantly increased in obese patients as compared with lean controls and decreased in approximately two-thirds of the participants post-intervention with a response that positively correlated with pre-intervention levels of this miR. Waist circumference, the inflammatory cytokine IL-8 and lipid parameters, principally total cholesterol, showed the strongest correlation with both the baseline levels and post-intervention correction of miR-146a-5p. Post-hoc analysis of experimental data supports the use of miR-146a-5p as a biomarker and predictor of the clinical response to physical activity in obese patients. Furthermore, miR-146a-5p expression was confirmed to increase together with that of the inflammatory genes TLR4, NF-κB, IL-6, and TNF-α in LPS-stimulated human mononuclear leukocytes. In conclusion, the inflamma-miR-146a-5p can serve as a personalized predictor of clinical outcome in obese patients entering physical activity weight-reduction programs.

Original languageEnglish
Pages (from-to)1012-1022
Number of pages11
JournalIUBMB Life
Volume70
Issue number10
DOIs
Publication statusPublished - Oct 1 2018

Fingerprint

MicroRNAs
Biomarkers
Exercise
Interleukin-8
Obesity
Interleukin-6
Weight Reduction Programs
Mononuclear Leukocytes
Genes
Cholesterol
Cytokines
Metabolic Diseases
Lipids
Plasmas
Waist Circumference
Clinical Trials
Therapeutics

Keywords

  • inflammation
  • lipid metabolism
  • metabolic syndrome
  • microRNA
  • obesity
  • physical activity
  • training exercise

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Clinical Biochemistry
  • Cell Biology

Cite this

Physical Activity Modulates the Overexpression of the Inflammatory miR-146a-5p in Obese Patients. / Russo, Angelo; Bartolini, Desireé; Mensà, Emanuela; Torquato, Pierangelo; Albertini, Maria Cristina; Olivieri, Fabiola; Testa, Roberto; Rossi, Sara; Piroddi, Marta; Cruciani, Gabriele; De Feo, Pierpaolo; Galli, Francesco.

In: IUBMB Life, Vol. 70, No. 10, 01.10.2018, p. 1012-1022.

Research output: Contribution to journalArticle

Russo, A, Bartolini, D, Mensà, E, Torquato, P, Albertini, MC, Olivieri, F, Testa, R, Rossi, S, Piroddi, M, Cruciani, G, De Feo, P & Galli, F 2018, 'Physical Activity Modulates the Overexpression of the Inflammatory miR-146a-5p in Obese Patients', IUBMB Life, vol. 70, no. 10, pp. 1012-1022. https://doi.org/10.1002/iub.1926
Russo, Angelo ; Bartolini, Desireé ; Mensà, Emanuela ; Torquato, Pierangelo ; Albertini, Maria Cristina ; Olivieri, Fabiola ; Testa, Roberto ; Rossi, Sara ; Piroddi, Marta ; Cruciani, Gabriele ; De Feo, Pierpaolo ; Galli, Francesco. / Physical Activity Modulates the Overexpression of the Inflammatory miR-146a-5p in Obese Patients. In: IUBMB Life. 2018 ; Vol. 70, No. 10. pp. 1012-1022.
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