Abstract
Specific microRNAs (miRs), including the “angio-miR-126” and the “inflamma-miR-146a-5p,” have been proposed as biomarkers and even therapeutic targets of obesity-associated metabolic diseases. Physical activity, a key measure of prevention for obesity and its complications, is reported to influence the expression of these miRs. In this study, we investigate whether a physical activity program proven to improve metabolic parameters in obese patients can correct the circulating levels of these miRs. Plasma miR-126 and miR-146a-5p were measured in a cohort of obese patients (n = 31, 16F + 15M) before and after the 3-month physical activity program of the CURIAMO trial (registration number for clinical trials: ACTRN12611000255987) and in 37 lean controls (24F + 13M). miR-146a-5p, but not miR-126, was significantly increased in obese patients as compared with lean controls and decreased in approximately two-thirds of the participants post-intervention with a response that positively correlated with pre-intervention levels of this miR. Waist circumference, the inflammatory cytokine IL-8 and lipid parameters, principally total cholesterol, showed the strongest correlation with both the baseline levels and post-intervention correction of miR-146a-5p. Post-hoc analysis of experimental data supports the use of miR-146a-5p as a biomarker and predictor of the clinical response to physical activity in obese patients. Furthermore, miR-146a-5p expression was confirmed to increase together with that of the inflammatory genes TLR4, NF-κB, IL-6, and TNF-α in LPS-stimulated human mononuclear leukocytes. In conclusion, the inflamma-miR-146a-5p can serve as a personalized predictor of clinical outcome in obese patients entering physical activity weight-reduction programs.
| Original language | English |
|---|---|
| Pages (from-to) | 1012-1022 |
| Number of pages | 11 |
| Journal | IUBMB Life |
| Volume | 70 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - Oct 1 2018 |
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Keywords
- inflammation
- lipid metabolism
- metabolic syndrome
- microRNA
- obesity
- physical activity
- training exercise
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Genetics
- Clinical Biochemistry
- Cell Biology
Cite this
Physical Activity Modulates the Overexpression of the Inflammatory miR-146a-5p in Obese Patients. / Russo, Angelo; Bartolini, Desireé; Mensà, Emanuela; Torquato, Pierangelo; Albertini, Maria Cristina; Olivieri, Fabiola; Testa, Roberto; Rossi, Sara; Piroddi, Marta; Cruciani, Gabriele; De Feo, Pierpaolo; Galli, Francesco.
In: IUBMB Life, Vol. 70, No. 10, 01.10.2018, p. 1012-1022.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Physical Activity Modulates the Overexpression of the Inflammatory miR-146a-5p in Obese Patients
AU - Russo, Angelo
AU - Bartolini, Desireé
AU - Mensà, Emanuela
AU - Torquato, Pierangelo
AU - Albertini, Maria Cristina
AU - Olivieri, Fabiola
AU - Testa, Roberto
AU - Rossi, Sara
AU - Piroddi, Marta
AU - Cruciani, Gabriele
AU - De Feo, Pierpaolo
AU - Galli, Francesco
PY - 2018/10/1
Y1 - 2018/10/1
N2 - Specific microRNAs (miRs), including the “angio-miR-126” and the “inflamma-miR-146a-5p,” have been proposed as biomarkers and even therapeutic targets of obesity-associated metabolic diseases. Physical activity, a key measure of prevention for obesity and its complications, is reported to influence the expression of these miRs. In this study, we investigate whether a physical activity program proven to improve metabolic parameters in obese patients can correct the circulating levels of these miRs. Plasma miR-126 and miR-146a-5p were measured in a cohort of obese patients (n = 31, 16F + 15M) before and after the 3-month physical activity program of the CURIAMO trial (registration number for clinical trials: ACTRN12611000255987) and in 37 lean controls (24F + 13M). miR-146a-5p, but not miR-126, was significantly increased in obese patients as compared with lean controls and decreased in approximately two-thirds of the participants post-intervention with a response that positively correlated with pre-intervention levels of this miR. Waist circumference, the inflammatory cytokine IL-8 and lipid parameters, principally total cholesterol, showed the strongest correlation with both the baseline levels and post-intervention correction of miR-146a-5p. Post-hoc analysis of experimental data supports the use of miR-146a-5p as a biomarker and predictor of the clinical response to physical activity in obese patients. Furthermore, miR-146a-5p expression was confirmed to increase together with that of the inflammatory genes TLR4, NF-κB, IL-6, and TNF-α in LPS-stimulated human mononuclear leukocytes. In conclusion, the inflamma-miR-146a-5p can serve as a personalized predictor of clinical outcome in obese patients entering physical activity weight-reduction programs.
AB - Specific microRNAs (miRs), including the “angio-miR-126” and the “inflamma-miR-146a-5p,” have been proposed as biomarkers and even therapeutic targets of obesity-associated metabolic diseases. Physical activity, a key measure of prevention for obesity and its complications, is reported to influence the expression of these miRs. In this study, we investigate whether a physical activity program proven to improve metabolic parameters in obese patients can correct the circulating levels of these miRs. Plasma miR-126 and miR-146a-5p were measured in a cohort of obese patients (n = 31, 16F + 15M) before and after the 3-month physical activity program of the CURIAMO trial (registration number for clinical trials: ACTRN12611000255987) and in 37 lean controls (24F + 13M). miR-146a-5p, but not miR-126, was significantly increased in obese patients as compared with lean controls and decreased in approximately two-thirds of the participants post-intervention with a response that positively correlated with pre-intervention levels of this miR. Waist circumference, the inflammatory cytokine IL-8 and lipid parameters, principally total cholesterol, showed the strongest correlation with both the baseline levels and post-intervention correction of miR-146a-5p. Post-hoc analysis of experimental data supports the use of miR-146a-5p as a biomarker and predictor of the clinical response to physical activity in obese patients. Furthermore, miR-146a-5p expression was confirmed to increase together with that of the inflammatory genes TLR4, NF-κB, IL-6, and TNF-α in LPS-stimulated human mononuclear leukocytes. In conclusion, the inflamma-miR-146a-5p can serve as a personalized predictor of clinical outcome in obese patients entering physical activity weight-reduction programs.
KW - inflammation
KW - lipid metabolism
KW - metabolic syndrome
KW - microRNA
KW - obesity
KW - physical activity
KW - training exercise
UR - http://www.scopus.com/inward/record.url?scp=85053456703&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85053456703&partnerID=8YFLogxK
U2 - 10.1002/iub.1926
DO - 10.1002/iub.1926
M3 - Article
VL - 70
SP - 1012
EP - 1022
JO - IUBMB Life
JF - IUBMB Life
SN - 1521-6543
IS - 10
ER -