Physical and functional independency of p70 and p58 natural killer (NK) cell receptors for HLA class I: Their role in the definition of different groups of alloreactive NK cell clones

Massimo Vitale, Simona Sivori, Daniela Pende, Raffaella Augugliaro, Carolina Di Donato, Antonio Amoroso, Mauro Malnati, Cristina Bottino, Lorenzo Moretta, Alessandro Moretta

Research output: Contribution to journalArticle

Abstract

Natural killer (NK) cells express clonally distributed receptors for different groups of HLA class I alleles. The Z27 monoclonal antibody described in this study recognizes a p70 receptor specific for HLA-B alleles belonging to the Bw4 supertypic specificity. Single amino acid substitutions in the peptide-binding groove of HLA-B2705 molecules influenced the recognition by some, but not all, p70/Z27+ clones. This suggests the existence of a limited polymorphism within the p70 family of receptors. The pattern of reactivity of monoclonal antibody Z27 revealed that Bw4-specific receptors may be expressed alone or in combination with different (GL183 and/or EB6) p58 molecules. Analysis of NK clones coexpressing p58 and p70 receptors allowed us to demonstrate that the two molecules represent physically and functionally independent receptors. The expression of p70 molecules either alone or in combination with EB6 molecules provided the molecular basis for understanding the cytolytic pattern of two previously defined groups of 'alloreactive' NK cell clones ('group 3' and 'group 5').

Original languageEnglish
Pages (from-to)1453-1457
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number4
DOIs
Publication statusPublished - Feb 20 1996

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KIR Receptors
Natural Killer Cells
Clone Cells
Alleles
Monoclonal Antibodies
HLA-B Antigens
Amino Acid Substitution
Peptides
p70 natural killer cell receptor

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

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title = "Physical and functional independency of p70 and p58 natural killer (NK) cell receptors for HLA class I: Their role in the definition of different groups of alloreactive NK cell clones",
abstract = "Natural killer (NK) cells express clonally distributed receptors for different groups of HLA class I alleles. The Z27 monoclonal antibody described in this study recognizes a p70 receptor specific for HLA-B alleles belonging to the Bw4 supertypic specificity. Single amino acid substitutions in the peptide-binding groove of HLA-B2705 molecules influenced the recognition by some, but not all, p70/Z27+ clones. This suggests the existence of a limited polymorphism within the p70 family of receptors. The pattern of reactivity of monoclonal antibody Z27 revealed that Bw4-specific receptors may be expressed alone or in combination with different (GL183 and/or EB6) p58 molecules. Analysis of NK clones coexpressing p58 and p70 receptors allowed us to demonstrate that the two molecules represent physically and functionally independent receptors. The expression of p70 molecules either alone or in combination with EB6 molecules provided the molecular basis for understanding the cytolytic pattern of two previously defined groups of 'alloreactive' NK cell clones ('group 3' and 'group 5').",
author = "Massimo Vitale and Simona Sivori and Daniela Pende and Raffaella Augugliaro and {Di Donato}, Carolina and Antonio Amoroso and Mauro Malnati and Cristina Bottino and Lorenzo Moretta and Alessandro Moretta",
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T2 - Their role in the definition of different groups of alloreactive NK cell clones

AU - Vitale, Massimo

AU - Sivori, Simona

AU - Pende, Daniela

AU - Augugliaro, Raffaella

AU - Di Donato, Carolina

AU - Amoroso, Antonio

AU - Malnati, Mauro

AU - Bottino, Cristina

AU - Moretta, Lorenzo

AU - Moretta, Alessandro

PY - 1996/2/20

Y1 - 1996/2/20

N2 - Natural killer (NK) cells express clonally distributed receptors for different groups of HLA class I alleles. The Z27 monoclonal antibody described in this study recognizes a p70 receptor specific for HLA-B alleles belonging to the Bw4 supertypic specificity. Single amino acid substitutions in the peptide-binding groove of HLA-B2705 molecules influenced the recognition by some, but not all, p70/Z27+ clones. This suggests the existence of a limited polymorphism within the p70 family of receptors. The pattern of reactivity of monoclonal antibody Z27 revealed that Bw4-specific receptors may be expressed alone or in combination with different (GL183 and/or EB6) p58 molecules. Analysis of NK clones coexpressing p58 and p70 receptors allowed us to demonstrate that the two molecules represent physically and functionally independent receptors. The expression of p70 molecules either alone or in combination with EB6 molecules provided the molecular basis for understanding the cytolytic pattern of two previously defined groups of 'alloreactive' NK cell clones ('group 3' and 'group 5').

AB - Natural killer (NK) cells express clonally distributed receptors for different groups of HLA class I alleles. The Z27 monoclonal antibody described in this study recognizes a p70 receptor specific for HLA-B alleles belonging to the Bw4 supertypic specificity. Single amino acid substitutions in the peptide-binding groove of HLA-B2705 molecules influenced the recognition by some, but not all, p70/Z27+ clones. This suggests the existence of a limited polymorphism within the p70 family of receptors. The pattern of reactivity of monoclonal antibody Z27 revealed that Bw4-specific receptors may be expressed alone or in combination with different (GL183 and/or EB6) p58 molecules. Analysis of NK clones coexpressing p58 and p70 receptors allowed us to demonstrate that the two molecules represent physically and functionally independent receptors. The expression of p70 molecules either alone or in combination with EB6 molecules provided the molecular basis for understanding the cytolytic pattern of two previously defined groups of 'alloreactive' NK cell clones ('group 3' and 'group 5').

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