Physical exercise activates the poly(ADP-ribosyl)ation system in rat testes

Maria Rosaria Faraone-Mennella, M. Marini, A. Ferone, O. Cacace, A. Liguoro, V. Margonato, B. Farina, A. Veicsteinas

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Given the high sensitivity of the male reproductive system to oxidative stress and to temperature changes, the amount of germ cell apoptosis and the activation of the poly(ADP-ribosyl)ation system (a very sensitive index of genotoxic stress) were evaluated in the testicular tissue of adult rats which underwent a 10-wk treadmill training, according to either a mild or a strong protocol; rats were sacrificed 24 h after the last training session or after a single bout of an additional stressing exercise (30 min of swimming). Controls were untrained rats (one resting group and one group with acute exercise). Both training and acute exercise increased marginally germ cell apoptotic indexes (caspase-induced poly(ADP-ribose) polymerase fragmentation and TUNEL-positive cells), while the activity of poly(ADP-ribose) polymerase and poly(ADP-ribose) glycohydrolase enzymes was affected in a way that suggests that acute exercise is associated with reversible genotoxic stress, and that training induces adaptive responses, as demonstrated by the activation of poly(ADP-ribose) polymerase system without subsequent increase in apoptosis.

Original languageEnglish
Pages (from-to)325-333
Number of pages9
JournalJournal of Biological Regulators and Homeostatic Agents
Volume24
Issue number3
Publication statusPublished - 2010

Fingerprint

Poly(ADP-ribose) Polymerases
Adenosine Diphosphate
Testis
Exercise
Germ Cells
DNA Damage
Apoptosis
In Situ Nick-End Labeling
Caspases
Oxidative Stress
Temperature
Enzymes

Keywords

  • Apoptosis
  • Physical exercise
  • Poly(ADP-ribosyl)ation
  • Rat testis

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Cancer Research
  • Immunology
  • Physiology (medical)
  • Endocrinology, Diabetes and Metabolism
  • Oncology
  • Immunology and Allergy
  • Medicine(all)

Cite this

Faraone-Mennella, M. R., Marini, M., Ferone, A., Cacace, O., Liguoro, A., Margonato, V., ... Veicsteinas, A. (2010). Physical exercise activates the poly(ADP-ribosyl)ation system in rat testes. Journal of Biological Regulators and Homeostatic Agents, 24(3), 325-333.

Physical exercise activates the poly(ADP-ribosyl)ation system in rat testes. / Faraone-Mennella, Maria Rosaria; Marini, M.; Ferone, A.; Cacace, O.; Liguoro, A.; Margonato, V.; Farina, B.; Veicsteinas, A.

In: Journal of Biological Regulators and Homeostatic Agents, Vol. 24, No. 3, 2010, p. 325-333.

Research output: Contribution to journalArticle

Faraone-Mennella, MR, Marini, M, Ferone, A, Cacace, O, Liguoro, A, Margonato, V, Farina, B & Veicsteinas, A 2010, 'Physical exercise activates the poly(ADP-ribosyl)ation system in rat testes', Journal of Biological Regulators and Homeostatic Agents, vol. 24, no. 3, pp. 325-333.
Faraone-Mennella MR, Marini M, Ferone A, Cacace O, Liguoro A, Margonato V et al. Physical exercise activates the poly(ADP-ribosyl)ation system in rat testes. Journal of Biological Regulators and Homeostatic Agents. 2010;24(3):325-333.
Faraone-Mennella, Maria Rosaria ; Marini, M. ; Ferone, A. ; Cacace, O. ; Liguoro, A. ; Margonato, V. ; Farina, B. ; Veicsteinas, A. / Physical exercise activates the poly(ADP-ribosyl)ation system in rat testes. In: Journal of Biological Regulators and Homeostatic Agents. 2010 ; Vol. 24, No. 3. pp. 325-333.
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