Physical map of the D6S149-D6S193 region on chromosome 6Q27 and its involement in benign surface epithelial ovarian tumours

Maria Grazia Tibiletti, Maurizio Trubia, Emanuela Ponti, Luca Sessa, Francesco Acquati, Daniela Furlan, Barbara Bernasconi, Manuela Fichera, Alessandra Mihalich, Andreas Ziegler, Armin Volz, Carla Facco, Cristina Riva, Laura Cremonesi, Maurizio Ferrari, Roberto Taramelli

Research output: Contribution to journalArticlepeer-review

Abstract

A detailed long range restriction map of the region defined by markers D6S149 and D6S193 on chromosome 6q27 has been constructed. This was achieved by YAC cloning and contig assembling of the same region. Seven YAC clones were found to span the almost 1000 Kb region flanked by the two markers which on the genetic map resulted to be 1.9 cM apart. With some of the characterized YAC clones we undertook a molecular cytogenetic analysis of 20 benign ovarian tumors. The rationale for this was the recent mapping to a region of chromosome 6q27, flanked by markers D6281 and D6S133, of a locus for the SV40-mediated immortalization of human cells (SEN6 gene). Noteworthy we found that the the D6S149-D6S193 region (comprised in the larger D6S281-D6S133 physical interval) was altered in all samples analysed adding support to the occurrence of a immortalization step in this type of tumors.

Original languageEnglish
Pages (from-to)1639-1642
Number of pages4
JournalOncogene
Volume16
Issue number12
Publication statusPublished - Mar 26 1998

Keywords

  • Benign ovarian tumors
  • Chromosome 6q27
  • Deletion
  • Physical map
  • YAC clones

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

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