Physico-chemical and toxicological characterization of iron-containing albumin nanoparticles as platforms for medical imaging

Ina Rosenberger, Christian Schmithals, Jennifer Vandooren, Silvia Bianchessi, Paolo Milani, Erica Locatelli, Liron L. Israel, Frank Hübner, Michela Matteoli, Jean Paul Lellouche, Mauro Comes Franchini, Lorena Passoni, Eugenio Scanziani, Ghislain Opdenakker, Albrecht Piiper, Jörg Kreuter

Research output: Contribution to journalArticle

Abstract

Iron oxide-containing magnetic nanoparticles (MNPs) have certain advantages over currently used contrast agents for tumor imaging by magnetic resonance imaging (MRI) as they offer the possibility of functionalization with ligands and tracers. Functionalized MNPs also may be used for targeted tumor therapy. In the current study nanoparticles (NPs) consisting of recombinant human serum albumin (rHSA) with incorporated hydrophilic (NH4)2Ce(IV)(NO3)6-γ-Fe2O3particles (CAN maghemite particles) for medical imaging were produced and characterized. For this purpose CAN maghemite particles were incorporated into an rHSA matrix to yield rHSA-NPs. The resulting NPs were analyzed by transmission electron microscopy, photon correlation spectroscopy, and atomic absorption. The sizes of the manufactured NP were 170 ± 10 nm, and the zeta-potential was - 50 ± 3 mV. The NPs remained stable when stored after lyophilization with sucrose 3% [w/v] as a cryoprotector. They showed pro-inflammatory properties without cell and animal toxicity in vivo and were highly contrasting in MRI. In conclusion, this report introduces novel rHSA NP with favorable properties containing iron oxide for detection by MRI.

Original languageEnglish
Pages (from-to)130-137
Number of pages8
JournalJournal of Controlled Release
Volume194
DOIs
Publication statusPublished - Nov 28 2014

Keywords

  • Air pouch model
  • Contrast agent
  • Diagnostic tool
  • Irwin test
  • Magnetic nanoparticles
  • Medical imaging

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Medicine(all)

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  • Cite this

    Rosenberger, I., Schmithals, C., Vandooren, J., Bianchessi, S., Milani, P., Locatelli, E., Israel, L. L., Hübner, F., Matteoli, M., Lellouche, J. P., Franchini, M. C., Passoni, L., Scanziani, E., Opdenakker, G., Piiper, A., & Kreuter, J. (2014). Physico-chemical and toxicological characterization of iron-containing albumin nanoparticles as platforms for medical imaging. Journal of Controlled Release, 194, 130-137. https://doi.org/10.1016/j.jconrel.2014.08.017