Physico-chemical properties of membranes of recovered erythrocytes in blood autologous transfusion: A study using fluorescence technique

R. Alleva, G. Ferretti, B. Borghi, E. Pignotti, A. Bassi, G. Curatola

Research output: Contribution to journalArticle

Abstract

Using fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene (DPH) and its derivative trimethylammonium -1,6-diphenyl-1,3,5-hexatriene (TMA-DPH), we have investigated the fluidity in erythrocyte membranes isolated from intraoperatively and postoperatively recovered erythrocytes, compared to membranes isolated from untreated erythrocytes or stored red blood cells (RBCs). Fluorescence polarization (Pf) of DPH and TMA-DPH was not significantly modified in membranes isolated from intraoperatively and postoperatively recovered RBCs compared to membranes isolated from untreated fresh RBCs or stored RBCs. However, using 2-dimethyl-amino-6-lauroyl-naphtalene (Laurdan), a very sensitive molecule, a significant increase in polarity in membranes from stored RBCs compared to untreated, intraoperatively and postoperatively recovered RBCs was observed. Moreover, there was significantly higher susceptibility to oxidative stress in membranes isolated from stored RBCs, compared to untreated and intraoperatively or postoperatively recovered RBC membranes, both at baseline and after oxidative stress. Our results suggest that intraoperative and postoperative recovered RBCs are less damaged than stored RBCs.

Original languageEnglish
Pages (from-to)291-297
Number of pages7
JournalTransfusion Science
Volume16
Issue number3
DOIs
Publication statusPublished - 1995

Fingerprint

Autologous Blood Transfusions
Erythrocyte Membrane
Erythrocytes
Fluorescence
Diphenylhexatriene
Membranes
Fluorescence Polarization
Oxidative Stress

ASJC Scopus subject areas

  • Immunology
  • Hematology

Cite this

Physico-chemical properties of membranes of recovered erythrocytes in blood autologous transfusion : A study using fluorescence technique. / Alleva, R.; Ferretti, G.; Borghi, B.; Pignotti, E.; Bassi, A.; Curatola, G.

In: Transfusion Science, Vol. 16, No. 3, 1995, p. 291-297.

Research output: Contribution to journalArticle

Alleva, R. ; Ferretti, G. ; Borghi, B. ; Pignotti, E. ; Bassi, A. ; Curatola, G. / Physico-chemical properties of membranes of recovered erythrocytes in blood autologous transfusion : A study using fluorescence technique. In: Transfusion Science. 1995 ; Vol. 16, No. 3. pp. 291-297.
@article{258d867187f146009053b37ed5316505,
title = "Physico-chemical properties of membranes of recovered erythrocytes in blood autologous transfusion: A study using fluorescence technique",
abstract = "Using fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene (DPH) and its derivative trimethylammonium -1,6-diphenyl-1,3,5-hexatriene (TMA-DPH), we have investigated the fluidity in erythrocyte membranes isolated from intraoperatively and postoperatively recovered erythrocytes, compared to membranes isolated from untreated erythrocytes or stored red blood cells (RBCs). Fluorescence polarization (Pf) of DPH and TMA-DPH was not significantly modified in membranes isolated from intraoperatively and postoperatively recovered RBCs compared to membranes isolated from untreated fresh RBCs or stored RBCs. However, using 2-dimethyl-amino-6-lauroyl-naphtalene (Laurdan), a very sensitive molecule, a significant increase in polarity in membranes from stored RBCs compared to untreated, intraoperatively and postoperatively recovered RBCs was observed. Moreover, there was significantly higher susceptibility to oxidative stress in membranes isolated from stored RBCs, compared to untreated and intraoperatively or postoperatively recovered RBC membranes, both at baseline and after oxidative stress. Our results suggest that intraoperative and postoperative recovered RBCs are less damaged than stored RBCs.",
author = "R. Alleva and G. Ferretti and B. Borghi and E. Pignotti and A. Bassi and G. Curatola",
year = "1995",
doi = "10.1016/0955-3886(95)00033-T",
language = "English",
volume = "16",
pages = "291--297",
journal = "Transfusion Science",
issn = "0955-3886",
publisher = "Pergamon Press Ltd.",
number = "3",

}

TY - JOUR

T1 - Physico-chemical properties of membranes of recovered erythrocytes in blood autologous transfusion

T2 - A study using fluorescence technique

AU - Alleva, R.

AU - Ferretti, G.

AU - Borghi, B.

AU - Pignotti, E.

AU - Bassi, A.

AU - Curatola, G.

PY - 1995

Y1 - 1995

N2 - Using fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene (DPH) and its derivative trimethylammonium -1,6-diphenyl-1,3,5-hexatriene (TMA-DPH), we have investigated the fluidity in erythrocyte membranes isolated from intraoperatively and postoperatively recovered erythrocytes, compared to membranes isolated from untreated erythrocytes or stored red blood cells (RBCs). Fluorescence polarization (Pf) of DPH and TMA-DPH was not significantly modified in membranes isolated from intraoperatively and postoperatively recovered RBCs compared to membranes isolated from untreated fresh RBCs or stored RBCs. However, using 2-dimethyl-amino-6-lauroyl-naphtalene (Laurdan), a very sensitive molecule, a significant increase in polarity in membranes from stored RBCs compared to untreated, intraoperatively and postoperatively recovered RBCs was observed. Moreover, there was significantly higher susceptibility to oxidative stress in membranes isolated from stored RBCs, compared to untreated and intraoperatively or postoperatively recovered RBC membranes, both at baseline and after oxidative stress. Our results suggest that intraoperative and postoperative recovered RBCs are less damaged than stored RBCs.

AB - Using fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene (DPH) and its derivative trimethylammonium -1,6-diphenyl-1,3,5-hexatriene (TMA-DPH), we have investigated the fluidity in erythrocyte membranes isolated from intraoperatively and postoperatively recovered erythrocytes, compared to membranes isolated from untreated erythrocytes or stored red blood cells (RBCs). Fluorescence polarization (Pf) of DPH and TMA-DPH was not significantly modified in membranes isolated from intraoperatively and postoperatively recovered RBCs compared to membranes isolated from untreated fresh RBCs or stored RBCs. However, using 2-dimethyl-amino-6-lauroyl-naphtalene (Laurdan), a very sensitive molecule, a significant increase in polarity in membranes from stored RBCs compared to untreated, intraoperatively and postoperatively recovered RBCs was observed. Moreover, there was significantly higher susceptibility to oxidative stress in membranes isolated from stored RBCs, compared to untreated and intraoperatively or postoperatively recovered RBC membranes, both at baseline and after oxidative stress. Our results suggest that intraoperative and postoperative recovered RBCs are less damaged than stored RBCs.

UR - http://www.scopus.com/inward/record.url?scp=0028868752&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028868752&partnerID=8YFLogxK

U2 - 10.1016/0955-3886(95)00033-T

DO - 10.1016/0955-3886(95)00033-T

M3 - Article

C2 - 10159887

AN - SCOPUS:0028868752

VL - 16

SP - 291

EP - 297

JO - Transfusion Science

JF - Transfusion Science

SN - 0955-3886

IS - 3

ER -