Abstract
Using fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene (DPH) and its derivative trimethylammonium -1,6-diphenyl-1,3,5-hexatriene (TMA-DPH), we have investigated the fluidity in erythrocyte membranes isolated from intraoperatively and postoperatively recovered erythrocytes, compared to membranes isolated from untreated erythrocytes or stored red blood cells (RBCs). Fluorescence polarization (Pf) of DPH and TMA-DPH was not significantly modified in membranes isolated from intraoperatively and postoperatively recovered RBCs compared to membranes isolated from untreated fresh RBCs or stored RBCs. However, using 2-dimethyl-amino-6-lauroyl-naphtalene (Laurdan), a very sensitive molecule, a significant increase in polarity in membranes from stored RBCs compared to untreated, intraoperatively and postoperatively recovered RBCs was observed. Moreover, there was significantly higher susceptibility to oxidative stress in membranes isolated from stored RBCs, compared to untreated and intraoperatively or postoperatively recovered RBC membranes, both at baseline and after oxidative stress. Our results suggest that intraoperative and postoperative recovered RBCs are less damaged than stored RBCs.
| Original language | English |
|---|---|
| Pages (from-to) | 291-297 |
| Number of pages | 7 |
| Journal | Transfusion Science |
| Volume | 16 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1995 |
Fingerprint
ASJC Scopus subject areas
- Immunology
- Hematology
Cite this
Physico-chemical properties of membranes of recovered erythrocytes in blood autologous transfusion : A study using fluorescence technique. / Alleva, R.; Ferretti, G.; Borghi, B.; Pignotti, E.; Bassi, A.; Curatola, G.
In: Transfusion Science, Vol. 16, No. 3, 1995, p. 291-297.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Physico-chemical properties of membranes of recovered erythrocytes in blood autologous transfusion
T2 - A study using fluorescence technique
AU - Alleva, R.
AU - Ferretti, G.
AU - Borghi, B.
AU - Pignotti, E.
AU - Bassi, A.
AU - Curatola, G.
PY - 1995
Y1 - 1995
N2 - Using fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene (DPH) and its derivative trimethylammonium -1,6-diphenyl-1,3,5-hexatriene (TMA-DPH), we have investigated the fluidity in erythrocyte membranes isolated from intraoperatively and postoperatively recovered erythrocytes, compared to membranes isolated from untreated erythrocytes or stored red blood cells (RBCs). Fluorescence polarization (Pf) of DPH and TMA-DPH was not significantly modified in membranes isolated from intraoperatively and postoperatively recovered RBCs compared to membranes isolated from untreated fresh RBCs or stored RBCs. However, using 2-dimethyl-amino-6-lauroyl-naphtalene (Laurdan), a very sensitive molecule, a significant increase in polarity in membranes from stored RBCs compared to untreated, intraoperatively and postoperatively recovered RBCs was observed. Moreover, there was significantly higher susceptibility to oxidative stress in membranes isolated from stored RBCs, compared to untreated and intraoperatively or postoperatively recovered RBC membranes, both at baseline and after oxidative stress. Our results suggest that intraoperative and postoperative recovered RBCs are less damaged than stored RBCs.
AB - Using fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene (DPH) and its derivative trimethylammonium -1,6-diphenyl-1,3,5-hexatriene (TMA-DPH), we have investigated the fluidity in erythrocyte membranes isolated from intraoperatively and postoperatively recovered erythrocytes, compared to membranes isolated from untreated erythrocytes or stored red blood cells (RBCs). Fluorescence polarization (Pf) of DPH and TMA-DPH was not significantly modified in membranes isolated from intraoperatively and postoperatively recovered RBCs compared to membranes isolated from untreated fresh RBCs or stored RBCs. However, using 2-dimethyl-amino-6-lauroyl-naphtalene (Laurdan), a very sensitive molecule, a significant increase in polarity in membranes from stored RBCs compared to untreated, intraoperatively and postoperatively recovered RBCs was observed. Moreover, there was significantly higher susceptibility to oxidative stress in membranes isolated from stored RBCs, compared to untreated and intraoperatively or postoperatively recovered RBC membranes, both at baseline and after oxidative stress. Our results suggest that intraoperative and postoperative recovered RBCs are less damaged than stored RBCs.
UR - http://www.scopus.com/inward/record.url?scp=0028868752&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028868752&partnerID=8YFLogxK
U2 - 10.1016/0955-3886(95)00033-T
DO - 10.1016/0955-3886(95)00033-T
M3 - Article
C2 - 10159887
AN - SCOPUS:0028868752
VL - 16
SP - 291
EP - 297
JO - Transfusion Science
JF - Transfusion Science
SN - 0955-3886
IS - 3
ER -