Physiologic oxygen enhances human embryonic stem cell clonal recovery and reduces chromosomal abnormalities

Nicholas R. Forsyth, Antonio Musio, Paolo Vezzoni, A. Hamish R W Simpson, Brendon S. Noble, Jim McWhir

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

Human embryonic stem cells (hESC) have great potential in regenerative medicine, provided that culture systems are established that maintain genomic integrity. Here we describe a comparison of the effects of culture in either physiologic oxygen (2%) or room oxygen (21%) on the hESC lines, H1, H9, and RH1. Physiologic oxygen enabled an average sixfold increase in clone recovery across the hESC lines tested (p <0.001). FACS analysis showed that cells cultured in physiologic oxygen were significantly smaller and less granular. No significant changes had occurred in levels of SSEA4, SSEA1, TRA-1-60, or TRA-1-81. While karyotypic normalcy was maintained in both H1 and H9, the frequency of spontaneous chromosomal aberrations was significantly increased in room oxygen. This increase was not observed in physiologic oxygen. These results clearly demonstrate that physiologic oxygen culture conditions are indispensable for robust hES clone recovery and may enhance the isolation of novel hES lines and transgenic clones.

Original languageEnglish
Pages (from-to)16-23
Number of pages8
JournalCloning and Stem Cells
Volume8
Issue number1
DOIs
Publication statusPublished - 2006

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Chromosome Aberrations
Oxygen
Clone Cells
Cell Line
Regenerative Medicine
Human Embryonic Stem Cells
Cultured Cells

ASJC Scopus subject areas

  • Biotechnology
  • Developmental Biology
  • Applied Microbiology and Biotechnology

Cite this

Physiologic oxygen enhances human embryonic stem cell clonal recovery and reduces chromosomal abnormalities. / Forsyth, Nicholas R.; Musio, Antonio; Vezzoni, Paolo; Simpson, A. Hamish R W; Noble, Brendon S.; McWhir, Jim.

In: Cloning and Stem Cells, Vol. 8, No. 1, 2006, p. 16-23.

Research output: Contribution to journalArticle

Forsyth, Nicholas R. ; Musio, Antonio ; Vezzoni, Paolo ; Simpson, A. Hamish R W ; Noble, Brendon S. ; McWhir, Jim. / Physiologic oxygen enhances human embryonic stem cell clonal recovery and reduces chromosomal abnormalities. In: Cloning and Stem Cells. 2006 ; Vol. 8, No. 1. pp. 16-23.
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