Physiological and biochemical characterization of trichoderma harzianum, a biological control agent against soilborne fungal plant pathogens

I. Grondona, R. Hermosa, M. Tejada, M. D. Gomis, P. F. Mateos, P. D. Bridge, E. Monte, I. Garcia-Acha

Research output: Contribution to journalArticle

Abstract

Monoconidial cultures of 15 isolates of Trichoderma harzianum were characterized on the basis of 82 morphological, physiological, and biochemical features and 99 isoenzyme bands from seven enzyme systems. The results were subjected to numerical analysis which revealed four distinct groups. Representative sequences of the internal transcribed spacer 1 (ITS 1)-ITS 2 region in the ribosomal DNA gene cluster were compared between groups confirming this distribution. The utility of the groupings generated from the morphological, physiological, and biochemical data was assessed by including an additional environmental isolate in the electrophoretic analysis. The in vitro antibiotic activity of the T. harzianum isolates was assayed against 10 isolates of five different soilborne fungal plant pathogens: Aphanomyces cochlioides, Rhizoctonia solani, Phoma betae, Acremonium eucurbitacearum, and Fusarium oxysporum f. sp. radicis lycopersici. Similarities between levels and specificities of biological activity and the numerical characterization groupings are both discussed in relation to antagonist-specific populations in known and potential biocontrol species.

Original languageEnglish
Pages (from-to)3189-3198
Number of pages10
JournalApplied and Environmental Microbiology
Volume63
Issue number8
Publication statusPublished - Aug 1997

Fingerprint

Biological Control Agents
Trichoderma
Trichoderma harzianum
biological control
plant pathogens
biological control agents
Aphanomyces
Aphanomyces cochlioides
pathogen
Fusarium oxysporum f. sp. radicis-lycopersici
Acremonium
Rhizoctonia
Phoma
Fusarium
Thanatephorus cucumeris
Multigene Family
Ribosomal DNA
multigene family
antibiotics
ribosomal DNA

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Grondona, I., Hermosa, R., Tejada, M., Gomis, M. D., Mateos, P. F., Bridge, P. D., ... Garcia-Acha, I. (1997). Physiological and biochemical characterization of trichoderma harzianum, a biological control agent against soilborne fungal plant pathogens. Applied and Environmental Microbiology, 63(8), 3189-3198.

Physiological and biochemical characterization of trichoderma harzianum, a biological control agent against soilborne fungal plant pathogens. / Grondona, I.; Hermosa, R.; Tejada, M.; Gomis, M. D.; Mateos, P. F.; Bridge, P. D.; Monte, E.; Garcia-Acha, I.

In: Applied and Environmental Microbiology, Vol. 63, No. 8, 08.1997, p. 3189-3198.

Research output: Contribution to journalArticle

Grondona, I, Hermosa, R, Tejada, M, Gomis, MD, Mateos, PF, Bridge, PD, Monte, E & Garcia-Acha, I 1997, 'Physiological and biochemical characterization of trichoderma harzianum, a biological control agent against soilborne fungal plant pathogens', Applied and Environmental Microbiology, vol. 63, no. 8, pp. 3189-3198.
Grondona, I. ; Hermosa, R. ; Tejada, M. ; Gomis, M. D. ; Mateos, P. F. ; Bridge, P. D. ; Monte, E. ; Garcia-Acha, I. / Physiological and biochemical characterization of trichoderma harzianum, a biological control agent against soilborne fungal plant pathogens. In: Applied and Environmental Microbiology. 1997 ; Vol. 63, No. 8. pp. 3189-3198.
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