TY - JOUR
T1 - Phytochemical profile of Juniperus oxycedrus ssp. oxycedrus berries
T2 - A new monoterpene glucoside and evaluation of the effects on cancer cell lines
AU - De Marino, Simona
AU - Festa, Carmen
AU - Zollo, Franco
AU - Rusolo, Fabiola
AU - Capone, Francesca
AU - Guerriero, Eliana
AU - Costantini, Susan
AU - De Felice, Vincenzo
AU - Iorizzi, Maria
PY - 2014
Y1 - 2014
N2 - Phytochemical investigation of polar extract from Juniperus oxycedrus spp. oxycedrus berries leds to the isolation of one new monoterpene glucoside (3R,6E)-3,7 dimethyl 8-hydroxy-6-octenoic acid 8-O-β-d-glucopyranoside along with seven known components, some of them were initially isolated from Juniperus communis L. berries. Their structures were established on the basis of extensive 1D and 2D NMR (1H, 13C, COSY, HMBC, HSQC, ROESY) and ESI-MS studies. The n-butanol fraction and isolated components, shikimic acid (2), compound 3, 4 and 5 were evaluated, in vitro, for their effect on cell viability against human malignant melanoma (A375), breast (MCF-7) and lung (H460) cancer cell lines. Shikimic acid exhibited selective effect on cell viability only against breast MCF-7 cell lines reaching IC50 value at dose of 30 μM and also induced the level decrease of vascular endothelial growth factor (VEGF) and five pro-inflammatory cytokines suggesting its potential anti-inflammatory effect.
AB - Phytochemical investigation of polar extract from Juniperus oxycedrus spp. oxycedrus berries leds to the isolation of one new monoterpene glucoside (3R,6E)-3,7 dimethyl 8-hydroxy-6-octenoic acid 8-O-β-d-glucopyranoside along with seven known components, some of them were initially isolated from Juniperus communis L. berries. Their structures were established on the basis of extensive 1D and 2D NMR (1H, 13C, COSY, HMBC, HSQC, ROESY) and ESI-MS studies. The n-butanol fraction and isolated components, shikimic acid (2), compound 3, 4 and 5 were evaluated, in vitro, for their effect on cell viability against human malignant melanoma (A375), breast (MCF-7) and lung (H460) cancer cell lines. Shikimic acid exhibited selective effect on cell viability only against breast MCF-7 cell lines reaching IC50 value at dose of 30 μM and also induced the level decrease of vascular endothelial growth factor (VEGF) and five pro-inflammatory cytokines suggesting its potential anti-inflammatory effect.
KW - Anti-inflammatory capacity
KW - Cytokinome profile
KW - Juniperus oxycedrus ssp. oxycedrus L.
KW - Monoterpene glucoside
KW - NMR
KW - Viability evaluation
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UR - http://www.scopus.com/inward/citedby.url?scp=84907801429&partnerID=8YFLogxK
U2 - 10.1016/j.phytol.2014.08.015
DO - 10.1016/j.phytol.2014.08.015
M3 - Article
AN - SCOPUS:84907801429
VL - 10
SP - 152
EP - 159
JO - Phytochemistry Letters
JF - Phytochemistry Letters
SN - 1874-3900
ER -