Phytochemical profile of Juniperus oxycedrus ssp. oxycedrus berries: A new monoterpene glucoside and evaluation of the effects on cancer cell lines

Simona De Marino, Carmen Festa, Franco Zollo, Fabiola Rusolo, Francesca Capone, Eliana Guerriero, Susan Costantini, Vincenzo De Felice, Maria Iorizzi

Research output: Contribution to journalArticlepeer-review

Abstract

Phytochemical investigation of polar extract from Juniperus oxycedrus spp. oxycedrus berries leds to the isolation of one new monoterpene glucoside (3R,6E)-3,7 dimethyl 8-hydroxy-6-octenoic acid 8-O-β-d-glucopyranoside along with seven known components, some of them were initially isolated from Juniperus communis L. berries. Their structures were established on the basis of extensive 1D and 2D NMR (1H, 13C, COSY, HMBC, HSQC, ROESY) and ESI-MS studies. The n-butanol fraction and isolated components, shikimic acid (2), compound 3, 4 and 5 were evaluated, in vitro, for their effect on cell viability against human malignant melanoma (A375), breast (MCF-7) and lung (H460) cancer cell lines. Shikimic acid exhibited selective effect on cell viability only against breast MCF-7 cell lines reaching IC50 value at dose of 30 μM and also induced the level decrease of vascular endothelial growth factor (VEGF) and five pro-inflammatory cytokines suggesting its potential anti-inflammatory effect.

Original languageEnglish
Pages (from-to)152-159
Number of pages8
JournalPhytochemistry Letters
Volume10
DOIs
Publication statusPublished - 2014

Keywords

  • Anti-inflammatory capacity
  • Cytokinome profile
  • Juniperus oxycedrus ssp. oxycedrus L.
  • Monoterpene glucoside
  • NMR
  • Viability evaluation

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science
  • Biochemistry
  • Biotechnology

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